FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Recombinant Construct: P{vas-Cas9(D10A).HA}
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General Information
Symbol
P{vas-Cas9(D10A).HA}
FlyBase ID
FBtp0131903
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9D10A.vas.Tag:HA,Tag:NLS(Unk)
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
Avic\GFPE.3xP3
Component allele Spyo\Cas9D10A.vas.Tag:HA,Tag:NLS(Unk)
Transgenic product class
Component
Use(s)
Regulatory region(s)
vas
Encoded product / tool
Cas9(D10A)
Tagged with
Tag:HA
Tag:NLS(Unk)
Description

The Cas9(D10A) nickase is expressed under the control of 5' and 3' vas regulatory elements. The Cas9(D10A) nickase is tagged with Tag:HA and a nuclear localization signal at the N-terminal end, and with a nuclear localization signal at the C-terminal end.

(Kurz et al., 2017)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P(3xP3-EGFP, vasa-cas9D10A)
(Kurz et al., 2017)
P{vas-Cas9(D10A).HA}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)