FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{U6:3-comt-Nsf2.gRNA.GGGG(FE)}
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General Information
Symbol
PBac{U6:3-comt-Nsf2.gRNA.GGGG(FE)}
FlyBase ID
FBtp0134089
Feature type
transgenic_transposable_element
Component Allele(s)
comtU6:3.gRNA.GGGG(FE)
Nsf2U6:3.gRNA.GGGG(FE)
Also Known As
gRNA-Nsf1-Nsf2
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele comtU6:3.gRNA.GGGG(FE)
Transgenic product class
sgRNA
(Poe et al., 2019)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
comt
Description

A snRNA:U6:96Ac promoter drives expression of four sgRNAs; two (AAAACGGTGGAGGTGCCCAG and GATGCCATTTGCAAGCAGCG) target comt for knockout and two (GAATGTGTCCGATTTCACGG and CCGCATCCTCGGTAACACGG) target Nsf2 for knockout. The sgRNAs are expressed as a polycistronic cassette based on the tRNA-gRNA design of FBrf0233589.

(Poe et al., 2019)
Component allele Nsf2U6:3.gRNA.GGGG(FE)
Transgenic product class
sgRNA
(Poe et al., 2019)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
Nsf2
Description

A snRNA:U6:96Ac promoter drives expression of four sgRNAs; two (AAAACGGTGGAGGTGCCCAG and GATGCCATTTGCAAGCAGCG) target comt for knockout and two (GAATGTGTCCGATTTCACGG and CCGCATCCTCGGTAACACGG) target Nsf2 for knockout. The sgRNAs are expressed as a polycistronic cassette based on the tRNA-gRNA design of FBrf0233589.

(Poe et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
81909
w*; PBac{U6:3-comt-Nsf2.gRNA.GGGG(FE)}VK00027
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
PBac{U6:3-comt-Nsf2.gRNA.GGGG(FE)}
(FlyBase, 1996-)
PBac{U6:3-comt-Nsf2.gRNA}
(FlyBase, 1992-)
gRNA-NSF1-NSF2
(Chen et al., 2024)
gRNA-NSF1-NSF2GGGG(FE)
(Koreman et al., 2021)
gRNA-Nsf1-Nsf2
(Poe et al., 2019)
Secondary FlyBase IDs
    References (6)