FB2026_03 , released September 17, 2026
Recombinant Construct: P{Hel25E.GFP}
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General Information
Symbol
P{Hel25E.GFP}
FlyBase ID
FBtp0137592
Feature type
transgenic_transposable_element
Component Allele(s)
Hel25EGFP
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Hel25EGFP
Transgenic product class
cDNA
(Meignin and Davis, 2008)
wild_type
(Meignin and Davis, 2008)
Component
Use(s)
Regulatory region(s)
Hel25E
Encoded product / tool
Hel25E
Tagged with
GFP
Description

The promoter and 5' regulatory sequences of Hel25E (673 bp) are fused with the Hel25E cDNA, without the stop codon (1270 bp). GFP is fused to the 3' of the cDNA followed by 1255 bp of the Hel25E 3' UTR.

(Meignin and Davis, 2008)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference

During oogenesis, Hel25EGFP is mainly nuclear but is also present in substantial quantities in the cytoplasm. During early embryogenesis, it is localized with DNA. At preblastoderm stages, it is associated with the fusome during metaphase. At the syncytial blastoderm stage, Hel25EGFP is nuclear with an accumulation at the basal pole of the nuclei at the same location as euchromatin.

(Meignin and Davis, 2008)
 
Marker for
Reflects expression of
Hel25E
(Meignin and Davis, 2008)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{Hel25E.GFP}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)