FlyBase Recombinant Construct Report: P{UASp-polo.K54M.RR.GFP}

UASp regulatory sequences drive expression of polo that carries a K54M amino acid replacement, resulting in a kinase-dead form of the protein. The coding sequence is tagged at the C-terminal end with GFP. Synonymous codon replacements have been introduced into the transgene, so that it is insensitive to RNAi mediated by P{GD7563}.

(Kachaner et al., 2017)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

polo^{K54M.UASp.RR.GFP}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Polo kin. dead-GFP

(Kachaner et al., 2017)

P{UASp-polo.K54M.GFP.RNAi-res}

(FlyBase, 1992-)

P{UASp-polo.K54M.RR.GFP}

(FlyBase, 1996-)

UASp-Polo^K54M::GFP

(Gallaud et al., 2022)

polo-KD-GFP

(Jones et al., 2024)

Secondary FlyBase IDs

References (6)

Report Sections

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