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General Information
Symbol
P{UAS-hpo.KD.VN}
FlyBase ID
FBtp0142318
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Component allele hpoKD.UAS.Tag:FLAG,N-Venus
Product class / Tool use(s)
Description

UASt regulatory sequences drive expression of a full-length hpo cDNA. The coding sequence has been mutated to carry a K71R mutation (resulting in a kinase-dead protein) and is tagged at the N-terminal end with Tag:FLAG and at the C-terminal end with N-Venus. Designed to be used in a bimolecular fluorescence complementation (BiFC) assay to study protein-protein interactions in vivo.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Molecular data

UASt regulatory sequences drive expression of a full-length hpo cDNA. The coding sequence has been mutated to carry a K71R mutation (resulting in a kinase-dead protein) and is tagged at the N-terminal end with Tag:FLAG and at the C-terminal end with N-Venus. Designed to be used in a bimolecular fluorescence complementation (BiFC) assay to study protein-protein interactions in vivo.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P{UAS-hpo.KD.VN}
UAS-hpoKDVN
UAS.HippoKinase-deadVenusN
Secondary FlyBase IDs
    References (4)