FB2026_02 , released June 18, 2026
Recombinant Construct: Mi{hsGFP}
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General Information
Symbol
Mi{hsGFP}
FlyBase ID
FBtp0144832
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
20 available
Molecular map
Description and Uses
Description

Mi{hsGFP} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with a cassette containing the fluorescent Avic\GFPHsp70.PD marker (donor sequence containing this cassette can be released from the P{FRT(attB.hsGFP.attB)} donor construct by FLP-mediated excision). Mi{hsGFP} is designed as an in vivo reporter for Polycomb group mediated gene silencing. It can be used to study silencing in the female germline, since the short Hsp70 5'UTR sequence present in the Avic\GFPHsp70.PD marker is designed to render the reporter insensitive to ovarian Hsp70-directed piRNAs.

(DeLuca et al., 2020)
Construct components
Component
Use(s)
Marker(s)
Avic\GFPHsp70.PD
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Mi{hsGFP}
(FlyBase, 1996-)
hsGFP
(DeLuca et al., 2020)
Secondary FlyBase IDs
    References (2)