FB2026_02 , released June 18, 2026
Recombinant Construct: PBac{αTub84B-dCas9::VPR,hh.gRNAx2}
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General Information
Symbol
PBac{αTub84B-dCas9::VPR,hh.gRNAx2}
FlyBase ID
FBtp0144914
Feature type
transgenic_transposable_element
Component Allele(s)
hhgRNAx2
Spyo\Cas9dCas9::VPR.αTub84B
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele hhgRNAx2
Transgenic product class
sgRNA
(Maselko et al., 2020)
Component
Use(s)
Encoded product / tool
hh
Description

Expresses two sgRNAs (the same as in P{TOE.GS00191}), both of which target the h promoter.

(Maselko et al., 2020)
Component allele Spyo\Cas9dCas9::VPR.αTub84B
Transgenic product class
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
dCas9::VPR
Description

A short αTub84B promoter drives expression of the dCas9::VPR transcriptional activator.

(Maselko et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{αTub84B-dCas9::VPR,hh.gRNAx2}
(FlyBase, 1996-)
Ptub::PTA::hh sgRNA
(Maselko et al., 2020)
Secondary FlyBase IDs
    References (3)