FB2026_02 , released June 18, 2026
Recombinant Construct: M{nanos-DD4-SpCas9}
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General Information
Symbol
M{nanos-DD4-SpCas9}
FlyBase ID
FBtp0145225
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9nanos.DD4
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9nanos.DD4
Transgenic product class
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
Spyo\Cas9
Tagged with
Tag:degron(ecDHFR)
Description

nos regulatory sequences (promoter and 3'UTR) regulate expression of Cas9, which has been tagged with three copies of the ligand-regulated Tag:degron(ecDHFR) destabilizing domain; one at the N-terminal end, one in an loop and the third at the C-terminal end. In the absence of trimethoprim (TMP), the expressed protein is rapidly degraded by the proteasome (due to the presence of Tag:degron(ecDHFR)). In the presence of TMP, only low Cas9 editing activity is seen.

(López Del Amo et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
M{nanos-DD4-SpCas9}
(FlyBase, 1996-)
M{nos-DD4-SpCas9}
(FlyBase, 1992-)
nos-DD4-SpCas9
(López Del Amo et al., 2020)
Secondary FlyBase IDs
    References (4)