FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{10XQUAS(FRT.stop)TrpA1-T2A-mCD8GFP}
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General Information
Symbol
P{10XQUAS(FRT.stop)TrpA1-T2A-mCD8GFP}
FlyBase ID
FBtp0145923
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFP10XQUAS.FRT.Tag:M(mCd8a)
TrpA110XQUAS.FRT
Size
Expression Data
Associated insertion(s)
3 available
Molecular map
Description and Uses
Construct components
Component allele TrpA110XQUAS.FRT
Transgenic product class
wild_type
(Potter, 2021.1.27)
Component
Use(s)
Regulatory region(s)
QUAS
Encoded product / tool
TrpA1
Also carries
FRT
FRT3
FRT5
Description

A transcriptional stop cassette that is flanked by three pairs of recombination sites (FRT, FRT3, FRT5) separates 10xQUAS from sequence that encodes two open reading frames (ORFs) separated by a T2A sequence (this allows each ORF to be translated separately). The two ORFs encode TrpA1 and Tag:M(mCd8a)-tagged GFP respectively. Excision of the stop cassette (using FLP recombinase) allows expression of the two ORFs.

(Potter, 2021.1.27)
Component allele Avic\GFP10XQUAS.FRT.Tag:M(mCd8a)
Transgenic product class
Component
Use(s)
Regulatory region(s)
QUAS
Encoded product / tool
GFP
Tagged with
Tag:M(mCd8a)
Also carries
FRT
FRT3
FRT5
Description

A transcriptional stop cassette that is flanked by three pairs of recombination sites (FRT, FRT3, FRT5) separates 10xQUAS from sequence that encodes two open reading frames (ORFs) separated by a T2A sequence (this allows each ORF to be translated separately). The two ORFs encode TrpA1 and Tag:M(mCd8a)-tagged GFP respectively. Excision of the stop cassette (using FLP recombinase) allows expression of the two ORFs.

(Potter, 2021.1.27)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{10XQUAS(FRT.stop)TrpA1-T2A-mCD8GFP}
(Potter, 2021.1.27, FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)