FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{U6:3-comt-Nsf2.gRNA.GVSQ(FE)}
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General Information
Symbol
PBac{U6:3-comt-Nsf2.gRNA.GVSQ(FE)}
FlyBase ID
FBtp0147645
Feature type
transgenic_transposable_element
Component Allele(s)
comtU6:3.gRNA.GVSQ(FE)
Nsf2U6:3.gRNA.GVSQ(FE)
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele comtU6:3.gRNA.GVSQ(FE)
Transgenic product class
sgRNA
(Koreman et al., 2021)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
comt
Description

A snRNA:U6:96Ac ( U6:3 ) promoter drives expression of a quadruple-gRNA in which a Drosophila tRNA-Gly (G) is followed by a gRNA targeting comt, and a tRNA-Val (V) is followed by a gRNA targeting Nsf2, and a tRNA-Ser (S) is followed by a second gRNA targeting Nsf2, and a tRNA-Gln (Q) is followed by a second gRNA targeting comt. This is all in conjunction with the (F+E) gRNA scaffold described in FBrf0241409.

(Koreman et al., 2021)
Component allele Nsf2U6:3.gRNA.GVSQ(FE)
Transgenic product class
sgRNA
(Koreman et al., 2021)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ac
Encoded product / tool
Nsf2
Description

A snRNA:U6:96Ac ( U6:3 ) promoter drives expression of a quadruple-gRNA in which a Drosophila tRNA-Gly (G) is followed by a gRNA targeting comt, and a tRNA-Val (V) is followed by a gRNA targeting Nsf2, and a tRNA-Ser (S) is followed by a second gRNA targeting Nsf2, and a tRNA-Gln (Q) is followed by a second gRNA targeting comt. This is all in conjunction with the (F+E) gRNA scaffold described in FBrf0241409.

(Koreman et al., 2021)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{U6:3-comt-Nsf2.gRNA.GVSQ(FE)}
(FlyBase, 1996-)
gRNA-NSF1-NSF2GVSQ(FE)
(Koreman et al., 2021)
Secondary FlyBase IDs
    References (3)