FlyBase Recombinant Construct Report: PBac{U6:3-sfl.gRNA.8R}

General Information

Symbol

PBac{U6:3-sfl.gRNA.8R}

FlyBase ID

FBtp0147659

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL80^{U6:3.gRNA(2.1)}

Scer\GAL80^Ubi-p63E.PK

sfl^U6:3.gRNA

Size

Expression Data

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele Scer\GAL80^{U6:3.gRNA(2.1)}

Transgenic product class

sgRNA

(Koreman et al., 2021)

Component

Use(s)

Regulatory region(s)

snRNA:U6:96Ac

Encoded product / tool

Scer\GAL80

Description

A snRNA:U6:96Ac ( U6:3 ) promoter drives expression a gRNA targeting GAL80. The 2.1 gRNA scaffold (which includes an additional extension of the second stem loop compared to the (F+E) gRNA scaffold described in FBrf0241409) is used.

(Koreman et al., 2021)

Component allele Scer\GAL80^Ubi-p63E.PK

Transgenic product class

Component

Use(s)

Regulatory region(s)

Ubi-p63E

Encoded product / tool

GAL80

binary expression system - repressor

Description

A Ubi-p63E promoter regulates expression of GAL80.

(Koreman et al., 2021)

Component allele sfl^U6:3.gRNA

Transgenic product class

sgRNA

(Koreman et al., 2021)

Component

Use(s)

Regulatory region(s)

snRNA:U6:96Ac

Encoded product / tool

sfl

Description

A snRNA:U6:96Ac ( U6:3 ) promoter drives expression of a triple-gRNA comprising 2 gRNAs targeting sfl and a gRNA targeting GAL80, each separated by a tRNA-Gly (G). The 2.1 gRNA scaffold (which includes an additional extension of the second stem loop compared to the (F+E) gRNA scaffold described in FBrf0241409) is used.

(Koreman et al., 2021)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments