FB2026_03 , released September 17, 2026
Recombinant Construct: P{UAS-bio.Sumo.2A.BirAopt}
Open Close
General Information
Symbol
P{UAS-bio.Sumo.2A.BirAopt}
FlyBase ID
FBtp0148374
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\birABirA(opt).UAS
SumoUAS.Tag:Bio(Unk)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Ecol\birABirA(opt).UAS
Transgenic product class
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
BirA(opt)
Description

UAS regulates expression of smt3 with an N-terminal Tag:Bio(Unk) tag, followed by a '2A' sequence, and then a human codon-optimized version of Ecol\birA. The resulting product is self-processed via 2A sequences to release the two separate proteins: the Ecol\birA enzyme recognizes the Tag:Bio(Unk) sequence, and transfers biotin to the lysine within the Tag:Bio(Unk) tag on smt3 .

(Pirone et al., 2017)
Component allele SumoUAS.Tag:Bio(Unk)
Transgenic product class
wild_type
(Pirone et al., 2017)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Sumo
Tagged with
Tag:Bio(Unk)
Description

UAS regulates expression of smt3 with an N-terminal Tag:Bio(Unk) tag, followed by a '2A' sequence, and then a human codon-optimized version of Ecol\birA. The resulting product is self-processed via 2A sequences to release the two separate proteins: the Ecol\birA enzyme recognizes the Tag:Bio(Unk) sequence, and transfers biotin to the lysine within the Tag:Bio(Unk) tag on smt3 .

(Pirone et al., 2017)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P{UAS-bio.Sumo.2A.BirAopt}
(FlyBase, 1996-)
P{UAS-bio.smt3.2A.BirAopt}
(FlyBase, 1996-)
bioSmt3
(Pirone et al., 2017)
p-bioSmt3
(Pirone et al., 2017)
Secondary FlyBase IDs
    References (4)