FlyBase Recombinant Construct Report: P{U6-tra.B.gRNA,U6-βTub85D.gRNA,Hsp-Cas9.T2A.GFP}

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Recombinant Construct: P{U6-tra.B.gRNA,U6-βTub85D.gRNA,Hsp-Cas9.T2A.GFP}

General Information

Symbol

P{U6-tra.B.gRNA,U6-βTub85D.gRNA,Hsp-Cas9.T2A.GFP}

FlyBase ID

FBtp0149840

Feature type

transgenic_transposable_element

Component Allele(s)

Avic\GFP^{EGFP.Hsp70Bb.K}

Spyo\Cas9^Hsp70Bb.PK

βTub85D^U6.sgRNA

Size

Expression Data

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component allele βTub85D^U6.sgRNA

Transgenic product class

(Akbari, 2018.7.9)

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Description

A snRNA:U6:96Ac promoter drives expression of a gRNA that targets βTub85D.

(Akbari, 2018.7.9)

Component allele tra^U6.sgRNA.B

Transgenic product class

(Kandul et al., 2019)

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Description

A snRNA:U6:96Ac promoter drives expression of a gRNA that targets tra.

(Kandul et al., 2019)

Component allele Spyo\Cas9^Hsp70Bb.PK

Transgenic product class

Component

Use(s)

Regulatory region(s)

Encoded product / tool

RNA-guided nuclease

Tagged with

nuclear protein localization tag

Description

A 476bp fragment encompassing the Hsp70Bb promoter drives expression of a 'Cas9.T2A.GFP' cassette, which consists of human codon-optimized Streptococcus pyogenes Cas9 (with a nuclear localization signal at each end) separated by a self-cleaving T2A motif from the EGFP open reading frame.

(Kandul et al., 2021)

Component allele Avic\GFP^{EGFP.Hsp70Bb.K}

Transgenic product class

Component

Use(s)

Regulatory region(s)

Encoded product / tool

green fluorescent protein

Description

A 476bp fragment encompassing the Hsp70Bb promoter drives expression of a 'Cas9.T2A.GFP' cassette, which consists of human codon-optimized Streptococcus pyogenes Cas9 (with a nuclear localization signal at each end) separated by a self-cleaving T2A motif from the EGFP open reading frame.

(Kandul et al., 2021)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^{EGFP.Hsp70Bb.K}

Phenotypic Class Phenotype Manifest In Detailed Description

Spyo\Cas9^Hsp70Bb.PK

Phenotypic Class Phenotype Manifest In Detailed Description

Phenotypic Class Phenotype Manifest In Detailed Description

βTub85D^U6.sgRNA

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

92796

w^*; P{U6-tra.B.gRNA,U6-βTub85D.gRNA,Hsp-Cas9.T2A.GFP}attP2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

P{U6-tra.B.gRNA,U6-βTub85D.gRNA,Hsp-Cas9.T2A.GFP}

(FlyBase, 1996-)

TI-pgSIT^{TraB,βTub,Hsp-Cas9}

(Kandul et al., 2021)

Secondary FlyBase IDs

References (3)

Report Sections