UASt regulatory sequences drive expression of full-length Hsap\TIAM1 isoform 1 cDNA (NM_001353694.2), containing the human disease-associated Arg23Cys variant.

UASt regulatory sequences drive expression of the Hsap\TIAM1 open reading frame. The endogenous stop codon is present, thus even though three copies of the Tag:HA tag are present downstream of the ORF, they are not expected to form part of the translated protein. The ORF (originally amplified from the 70631 clone) has been mutated to carry p.R23C. The human ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or tag sequence.