FB2026_03 , released September 17, 2026
Recombinant Construct: P{nanos-Cas9.H840A}
Open Close
General Information
Symbol
P{nanos-Cas9.H840A}
FlyBase ID
FBtp0159111
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9H840A.nanos
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9H840A.nanos
Transgenic product class
missense_variant
(Ren et al., 2014)
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
Spyo\Cas9
Description

nanos regulatory sequences (~700bp promoter sequence, the 5'UTR and the 3'UTR) regulate expression of Spyo\Cas9 carrying a H840A amino acid replacement. This mutation is predicted to inactivate the HNH domain and should result in the encoded protein acting as a nickase that cleaves a single DNA strand when targeted to a genomic site of interest using sgRNA, however only very low levels of mutagenesis were seen when this transgene was used in combination with two sgRNAs targeting a region of interest.

(Ren et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{nanos-Cas9.H840A}
(FlyBase, 1992-)
nos-Cas9H840A
(Ren et al., 2014)
Secondary FlyBase IDs
    References (3)