FB2026_02 , released June 18, 2026
Recombinant Construct: P{UAS-shi.gRNA-target}
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General Information
Symbol
P{UAS-shi.gRNA-target}
FlyBase ID
FBtp0159142
Feature type
transgenic_transposable_element
Component Allele(s)
shiUAS.gRNA-target
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele shiUAS.gRNA-target
Transgenic product class
silent_mutation
(Chen et al., 2020)
wild_type
(Chen et al., 2020)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
shi
Description

10xUAS regulatory sequences drive expression of codon-optimized shi coding sequence that also carries silent mutations that maintain the translated amino acid sequence but creates a sequence that is the target of the gRNA encoded by P{U6-shi.gRNA} (this gRNA does not target the endogenous shi gene). The gRNA target site is in the same region of the coding sequence as the lesion in shi1.

(Chen et al., 2020)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-shi.gRNA-target}
(FlyBase, 1992-)
UAS-shibire
(Chen et al., 2020)
Secondary FlyBase IDs
    References (3)