FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: PBac{UAS-scSPT.H59L.HA}
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General Information
Symbol
PBac{UAS-scSPT.H59L.HA}
FlyBase ID
FBtp0160334
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\SPTLC1UAS.Tag:HA
Hsap\SPTLC2UAS.Tag:HA
Hsap\SPTSSAH59L.UAS.Tag:HA
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

 :UAS regulates expression of a "single chain SPT" (scSPT) that expresses the three human SPT subunits (encoded by Hsap\SPTLC2, Hsap\SPTSSA and Hsap\SPTLC1), tagged with Tag:HA. The Hsap\SPTSSA gene contains a H59L substitution.

(Srivastava et al., 2023)
Construct components
Component allele Hsap\SPTSSAH59L.UAS.Tag:HA
Transgenic product class
missense_variant
(Srivastava et al., 2023)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\SPTSSA
Tagged with
Tag:HA
Description

UAS regulates expression of Hsap\SPTSSA with a H59L mutation, in the context of a "UAS-scSPT" construct that encodes "single chain SPT" (scSPT) expressing the three human SPT subunits (encoded by Hsap\SPTLC2, Hsap\SPTSSA and Hsap\SPTLC1), tagged with Tag:HA. Hsap\SPTSSA H59L is analogous to the mouse 'Sptssb' p.H56L variant that causes elevated sphingolipid synthesis and neurodegeneration.

(Srivastava et al., 2023)
Component allele Hsap\SPTLC2UAS.Tag:HA
Transgenic product class
wild_type
(Srivastava et al., 2023)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\SPTLC2
Tagged with
Tag:HA
Description

UAS regulates expression of wild type Hsap\SPTLC2 in the context of a "UAS-scSPT" construct that encodes "single chain SPT" (scSPT) expressing the three human SPT subunits (encoded by Hsap\SPTLC2, Hsap\SPTSSA and Hsap\SPTLC1), tagged with Tag:HA.

(Srivastava et al., 2023)
Component allele Hsap\SPTLC1UAS.Tag:HA
Transgenic product class
wild_type
(Srivastava et al., 2023)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\SPTLC1
Tagged with
Tag:HA
Description

UAS regulates expression of wild type Hsap\SPTLC1 in the context of a "UAS-scSPT" construct that encodes "single chain SPT" (scSPT) expressing the three human SPT subunits (encoded by Hsap\SPTLC2, Hsap\SPTSSA and Hsap\SPTLC1), tagged with Tag:HA.

(Srivastava et al., 2023)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{UAS-scSPT.H59L.HA}
(FlyBase, 1996-)
scSPTH59L
(Srivastava et al., 2023)
Secondary FlyBase IDs
    References (3)