FB2026_02 , released June 18, 2026
Recombinant Construct: P{UAS-Doa.227kD.RNAi}
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General Information
Symbol
P{UAS-Doa.227kD.RNAi}
FlyBase ID
FBtp0160809
Feature type
transgenic_transposable_element
Component Allele(s)
DoaRNAi.UAS.227kD
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele DoaRNAi.UAS.227kD
Transgenic product class
RNAi_reagent
(Rabinow, 2023.6.12)
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Doa
Description

UASt regulatory sequences drive expression of a dsRNA that targets Doa transcripts RP and RV (these encode the 227kD Doa protein isoform). The sequence used to create the inverted repeat is TTCTAGAGGGTATATGAGTTCGACTAGAAGTCAGCAATGGAGCTCCCATGGGTTGACCACCACCCGGCACATGCGTCATGGACATGCTCTTTGGAAGCACCTGTTGCTGCTGTGCTGGAAATTGCTGCTTCACAGGCACTCTACTCACAAAATTCGATCCTGCCAACGGCGATTGCTCGTTGCAGATCAACTTATAGAGATCGTCCTTGGTCACCGCCTGACGCACCTTGAGATCCAGGTGCGACAAAGATGCATTTTTCTGCAGTTTGTGGGGCTGATTTTGGGGCACCTGTGACTGCTGAGGTCGCTGCAGCTCCTTCTGAGCGGCCTCCGCAACTGTGGGATCAAATATGAATTC.

(Rabinow, 2023.6.12)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
99682
w*; CyO/wgSp-1; P{UAS-Doa.227kD.RNAi}1/TM6B, Tb1
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{UAS-Doa.227kD.IR}
(Rabinow, 2023.6.12, FlyBase, 1992-)
P{UAS-Doa.227kD.RNAi}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (4)