FB2026_02 , released June 18, 2026
Recombinant Construct: P{UAS-pHluorinSE-mKate2-KDEL-V5}
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General Information
Symbol
P{UAS-pHluorinSE-mKate2-KDEL-V5}
FlyBase ID
FBtp0161822
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Description

UASt regulatory sequences drive expression of an 'ER-phagy' sensor designed for analysis of the dynamics of endoplasmic reticulum (ER) autophagic flux. The sensor consists of a Tag:SS(BiP) signal sequence, the pHluorinSE (pH-sensitive) and mKate2 (pH-resistant) fluorescent proteins, a Tag:ER(KDEL) ER retention signal and the Tag:V5 epitope. Under normal conditions, fluorescence from both pHluorinSE and mKate2 occurs. Upon clearance of endoplasmic reticulum (ER) by 'ER-phagy', in which ER fragments are delivered to autophagosomes and then fuse with lysosomes, the low pH of the lysosome quenches the pHluorinSE signal but not the mKate2 signal.

(Wang et al., 2023)
Construct components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
mKate2
pHluorinSE
Tagged with
Tag:ER(KDEL)
Tag:SS(BiP)
Tag:V5
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
P{UAS-pHluorinSE-mKate2-KDEL-V5}
(FlyBase, 1996-)
UAS-ss-pHluorin-mKate2-KDEL-V5
(Wang et al., 2023)
UAS::ss-pHluorin-mKate2-KDEL-V5
(Wang et al., 2023)
ss-PK-KDEL
(Wang et al., 2023)
Secondary FlyBase IDs
    References (2)