PBac{SB-LT} encodes Spyo\Cas9dCas9::VPR.αTub84B.long.Tag:FLAG and a ubiquitously expressed sgRNA that targets eve.
A long αTub84B promoter fragment drives expression of the dCas9::VPR transcriptional activator. The driver is tagged at the N-terminus with 3xTag:FLAG and a nuclear localization signal.