FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{DB1-LT}
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General Information
Symbol
P{DB1-LT}
FlyBase ID
FBtp0167693
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9dCas9::VPR.αTub84B.long.Tag:FLAG
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

P{DB1-LT} encodes Spyo\Cas9dCas9::VPR.αTub84B.long.Tag:FLAG and two ubiquitously expressed sgRNAs (that target eve and hid respectively).

(Waters et al., 2018)
Construct components
Component allele Spyo\Cas9dCas9::VPR.αTub84B.long.Tag:FLAG
Transgenic product class
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
dCas9::VPR
Tagged with
Tag:FLAG
Tag:NLS(Unk)
Description

A long αTub84B promoter fragment drives expression of the dCas9::VPR transcriptional activator. The driver is tagged at the N-terminus with 3xTag:FLAG and a nuclear localization signal.

(Waters et al., 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{DB1-LT}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)