FB2026_03 , released September 17, 2026
Recombinant Construct: P{nanos-xCas9(3.7)-BE3}
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General Information
Symbol
P{nanos-xCas9(3.7)-BE3}
FlyBase ID
FBtp0168338
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9xCas9(3.7)-BE3.nanos
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9xCas9(3.7)-BE3.nanos
Transgenic product class
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
Spyo\Cas9
Description

The nanos promoter drives expression of 'xCas9(3.7)-BE3', a base editor that targets cytosine in DNA; it favors the conversion of C to T. The xCas9(3.7)-BE3 sequence (from Addgene plasmid 108380) encodes a fusion protein composed of 1. a modified rat Apobec1 cytidine deaminase ( RGDID:2133 ), 2. x-Cas9(3.7), an engineered Cas9 nickase (contains the D10A mutation) that contains mutations broadening the PAM compatibility to GAA, GAT, and NG in addition to its canonical NGG, and 3. the uracil DNA glycosylase inhibitor (UGI) from a Bacillus subtilis bacteriophage (see PMID:29512652).

(Thakkar et al., 2023)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{nanos-xCas9(3.7)-BE3}
(FlyBase, 1992-)
nos-BE3
(Thakkar et al., 2023)
Secondary FlyBase IDs
    References (3)