FB2026_02 , released June 18, 2026
Recombinant Construct: P{GTL3.roX1}
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General Information
Symbol
P{GTL3.roX1}
FlyBase ID
FBtp0169952
Feature type
transgenic_transposable_element
Component Allele(s)
lncRNA:roX1U6.GTL3
Spyo\Cas9dCas9.Act5C.Tag:FLAG
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9dCas9.Act5C.Tag:FLAG
Transgenic product class
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
dCas9(D10A.H840A)
Tagged with
Tag:FLAG
Tag:NLS(SV40-largeT)
Description

The Act5C promoter drives expression of dCas9(D10A.H840A) that is codon optimized for Drosophila. The coding sequence is tagged at the N-terminal end with 3xTag:FLAG and Tag:NLS(SV40-largeT) and at the C-terminal end with a Tag:NLS(SV40-largeT).

(Ghosh et al., 2016)
Component allele lncRNA:roX1U6.GTL3
Transgenic product class
sgRNA
(Ghosh et al., 2016)
Component
Use(s)
Encoded product / tool
lncRNA:roX1
Description

Constitutively expresses two sgRNAs (AAGAAAAGTGTTAGTTACC and TCGACAAGTGGCAGCCCTAA), each of which target lncRNA:roX1.

(Ghosh et al., 2016)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{GTL3.roX1}
(FlyBase, 1996-)
pGTL-3-roX1
(Ghosh et al., 2016)
rT8+rT9-Actin:dCas9
(Ghosh et al., 2016)
Secondary FlyBase IDs
    References (3)