FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{vas-Cas9.S.GFP-}
Open Close
General Information
Symbol
M{vas-Cas9.S.GFP-}
FlyBase ID
FBtp0170451
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9vas.Tag:NLS(SV40-largeT)
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Description

Transgene in which the Avic\GFPE.3xP3 marker present in M{vas-Cas9.S} has been inactivated using a gRNA that targets EGFP. The resulting M{vas-Cas9.S.GFP-} transgene is marked only with Disc\RFP3xP3.PB.

(Sebo et al., 2014)
Construct components
Component
Use(s)
Marker(s)
Disc\RFP3xP3.PB
Component allele Spyo\Cas9vas.Tag:NLS(SV40-largeT)
Transgenic product class
Component
Use(s)
Regulatory region(s)
vas
Encoded product / tool
Cas9
Tagged with
Tag:NLS(SV40-largeT)
Description

vas regulatory sequences drive expression of human codon-optimized Cas9 endonuclease tagged with the Tag:NLS(SV40-largeT) nuclear localization signal.

(Sebo et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
M{vas-Cas9.S}
(Sebo et al., 2014)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
605940
y1 M{vas-Cas9.S.GFP-}ZH-2A w1118
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
M{vas-Cas9.S.GFP-}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (3)