Mi{T2A-GAL4.1} represents a transgenic construct generated in vivo by using phiC31:int-mediated recombination to replace the attP cassette of a Mi{MIC} element insertion with an intron phase 1 cassette composed of a splice acceptor site, the T2A peptide, the GAL4 coding sequence, a stop codon and the Hsp70 3'UTR.