FB2026_03 , released September 17, 2026
Recombinant Construct: P{FRT(24xMS2-V6)}
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General Information
Symbol
P{FRT(24xMS2-V6)}
FlyBase ID
FBtp0172970
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

P{FRT(24xMS2-V6)} is a transgenic donor construct containing a recombination-mediated cassette exchange (RMCE) cassette flanked by FRT sites. The RMCE cassette consists of two inverted attB sites which surround a '24xMS2-V6' stem-loop cassette that contains 24 copies of Tag:MS2 (the cassette contains 2 tandem copies of a sequence encoding 12 unique Tag:MS2 stem loops). Each copy of Tag:MS2 contains a U instead of C at a crucial nucleotide in the Tag:MCP binding site that weakens the interaction and makes the Tag:MS2-Tag:MCP interaction less stable. A w+mW.hs marker is located outside of the RMCE cassette, between one of the attB sites and one of the FRT sites. Upon expression of FLP recombinase, the RMCE cassette is released as a circular DNA, which in the presence of phiC31:int integrase can undergo RMCE with a target site containing inverted attP sites, replacing the target 'inverted attP' cassette with the 24xMS2-V6 stem-loop cassette.

(Beadle et al., 2024)
Construct components
Component
Use(s)
Element type
Tagged with
Tag:MS2
Also carries
attB
FRT
Marker(s)
w+mW.hs
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{FRT(24xMS2-V6)}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)