FB2026_03 , released September 17, 2026
Recombinant Construct: P{CoinFLP-Act5C-FLAG::Cas9(R691A)-T2A-H2B-GFP.-FRT}
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General Information
Symbol
P{CoinFLP-Act5C-FLAG::Cas9(R691A)-T2A-H2B-GFP.-FRT}
FlyBase ID
FBtp0172982
Feature type
transgenic_transposable_element
Component Allele(s)
Spyo\Cas9R691A.Act5C.CoinFLP-FRT
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Spyo\Cas9R691A.Act5C.CoinFLP-FRT
Transgenic product class
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
Cas9(R691A)
Tagged with
Tag:FLAG
Description

The FRT cassette has been excised from P{CoinFLP-Act5C-FLAG::Cas9(R691A)-T2A-H2B-GFP}, removing the transcriptional stop sequence and allowing the Act5C regulatory sequences to drive expression of a coding sequence that encodes two open reading frames separated by a T2A linker (this allows for separate translation of each protein). The first ORF encodes Cas9(R691A) tagged with Tag:FLAG. The second ORF consists of Histone2B tagged with GFP.

(Ochi et al., 2024)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
P{CoinFLP-Act5C-FLAG::Cas9(R691A)-T2A-H2B-GFP.-FRT}
(FlyBase, 1992-)
Secondary FlyBase IDs
    References (3)