FB2026_03 , released September 17, 2026
FB2026_03 , released September 17, 2026
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Term split luciferase complementation ID (Ontology) MI:1203 (Molecular Interactions)
Definition Two proteins of interest, a bait and prey, which are genetically fused to amino- and carboxy-terminal fragments of luciferase, are transiently expressed. Physical interactions of these bait and prey proteins reconstitute some of the luciferase activity and result in light emission in the presence of the luciferase substrate.[ PubMed:20734273 ]
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 split luciferase complementation (all annotations which use CV term, excluding "NOT" statements)
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  experimental interaction detection
   |__protein complementation assay
       |__split luciferase complementation  59 rec.
           |__split firefly luciferase complementation
           |__Split renilla luciferase complementation
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Is a protein complementation assay
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