Allele Dmel\scb²

General Information
Symbol	Dmel\scb²	Species	D. melanogaster
Name		FlyBase ID	FBal0015261
Feature type	allele	Associated gene	Dmel\scb
Also Known As	scb^IIG

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Tearle and Nusslein-Volhard, 1987)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	dorsal superficial trachea (Stark et al., 1997) embryo \| germline clone (Schock and Perrimon, 2003) embryo \| germline clone (with Df(2R)Jp1) (Schock and Perrimon, 2003) embryo \| germline clone (with scb^5J38) (Schock and Perrimon, 2003) embryonic/larval salivary gland \| embryonic stage (Stark et al., 1997) embryonic heart cardioblast (Vanderploeg et al., 2012) extended germ band embryo (Stark et al., 1997) germ band \| germline clone (Schock and Perrimon, 2003) germ band \| germline clone (with Df(2R)Jp1) (Schock and Perrimon, 2003) germ band \| germline clone (with scb^5J38) (Schock and Perrimon, 2003) pericardial cell (Stark et al., 1997)
Detailed Description
	Statement Reference The cardioblasts of the heart region of the dorsal vessel in homozygous stage embryos show a less regular alignment of their nuclei along the midline in mutant embryos compared to wild type. No lumen develops between contralaterally apposed cardioblasts in the heart region of mutant embryos, in contrast to wild type. The leading edge membrane of migrating mutant cardioblasts shows less vigorous activity than that of wild-type cardioblasts. (Vanderploeg et al., 2012) Mutant embryos have no severe defects in salivary gland shape or positioning. (Bradley et al., 2003) Germ-line clones exhibit a U shaped embryonic phenotype at low penetrance (about 4.5%) suggesting germ band retraction defects. scb^5J38/scb² embryos also exhibit a U shaped embryonic phenotype at low penetrance (about 4%). scb²/Df(2R)Jp1 show the same phenotype at 11% penetrance. (Schock and Perrimon, 2003) The germ band twists laterally, rather than extending dorsally as in wild type. Proper orientation of the germ band in recovered by the completion of germ-band extension. Embryos exhibit mislocalisation of the pericardial cells and there are fewer of these cells than in wild type. Embryos exhibit significant gaps in the dorsal trunk of the trachea. Embryos frequently display one salivary gland misshapen and smaller than the other, the gland may also be shifted closer to the midline. (Stark et al., 1997)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference scb²/scb[+] is an enhancer of visible \| dominant phenotype of sog^EP7 (Araujo et al., 2003) scb²/scb[+] is an enhancer of visible \| dominant phenotype of sog^EP11 (Araujo et al., 2003)
NOT Suppressor of
Statement Reference scb² is a non-suppressor of lethal \| pupal stage phenotype of Csk^j1D8/Csk^S030003 (Read et al., 2004)
Other
Statement Reference Fak^N30/Fak^KG00304, scb²/scb[+] has neuroanatomy defective \| third instar larval stage phenotype (Tsai et al., 2008)
Phenotype Manifest In
Enhanced by
Statement Reference scb² has embryo \| germline clone phenotype, enhanceable by wb^4Y18 (Schock and Perrimon, 2003) scb² has germ band \| germline clone phenotype, enhanceable by wb^4Y18 (Schock and Perrimon, 2003)
Enhancer of
Statement Reference scb²/scb[+] is an enhancer of wing vein phenotype of sog^EP7 (Araujo et al., 2003) scb²/scb[+] is an enhancer of wing vein phenotype of sog^EP11 (Araujo et al., 2003) scb² is an enhancer of embryo \| germline clone phenotype of wb^4Y18 (Schock and Perrimon, 2003) scb² is an enhancer of germ band \| germline clone phenotype of wb^4Y18 (Schock and Perrimon, 2003)
Suppressor of
Statement Reference scb²/scb[+] is a suppressor of eye phenotype of CycE^JP (Brumby et al., 2004) scb² is a suppressor of eye disc & S phase phenotype of CycE^JP (Brumby et al., 2004)
Other
Statement Reference Dab^M54-R, scb²/scb[+] has embryonic heart cardioblast phenotype (Vanderploeg et al., 2012) Fak^N30/Fak^KG00304, scb²/scb[+] has neuromuscular junction \| third instar larval stage phenotype (Tsai et al., 2008) Fak^N30/Fak^KG00304, scb²/scb[+] has NMJ bouton \| supernumerary \| third instar larval stage phenotype (Tsai et al., 2008) LanA^9-32, scb²/scb[+] has embryonic heart cardioblast phenotype (Vanderploeg et al., 2012) mew^M6, scb² has embryonic/larval midgut \| embryonic stage phenotype (Martin-Bermudo et al., 1999, Martin-Bermudo et al., 1999) mys¹, scb²/scb[+] has embryonic heart cardioblast phenotype (Vanderploeg et al., 2012) rhea¹, scb²/scb[+] has embryonic heart cardioblast phenotype (Vanderploeg et al., 2012) scb²/scb[+], vkg^p1003-83 has embryonic heart cardioblast phenotype (Vanderploeg et al., 2012) scb²/scb[+], wb^SF11 has embryonic heart cardioblast phenotype (Vanderploeg et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference mys[1]/+ ; scb[2]/+ and vkg[p1003-83]/scb[2] double heterozygous embryos show defects in continuity and alignment of the cardioblasts at the dorsal midline. LanA[9-32]/+ ; scb[2]/+ and wb[SF11]/scb[2] double heterozygous embryos show defects in alignment of the cardioblasts at the dorsal midline. (Vanderploeg et al., 2012) Significant overgrowth of the neuromuscular junction (NMJ) (increased bouton number per muscle area and increased NMJ length per muscle area) is seen in scb[2]/+ Fak[N30]/Fak[KG00304] third instar larvae. (Tsai et al., 2008) Suppresses the S phase defects seen in the CycE^JP eye disc. (Brumby et al., 2004) Salivary glands in mew^M6 scb² mutant embryos are indistinguishable from those in mew^M6 embryos. (Bradley et al., 2003) Heterozygosity for scb² does not result in lethality in Abl¹/+ embryos derived from homozygous Abl¹ female germline clones. (Grevengoed et al., 2001) if^B4 scb² double mutant embryos show no difference in midgut development compared to if^B4 single mutant embryos. mew^M6 scb² double mutant embryos show a strong defect in the migration of the endodermal midgut cells, with a delay in migration of approximately 2 hours (similar to that observed in embryos lacking both maternal and zygotic mys function). (Martin-Bermudo et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	scb^62S9 (Brumby et al., 2004) scb^E93 (Brumby et al., 2004)
Partially rescued by	scb² is partially rescued by scb^Scer\UAS.cVa/Scer\GAL4^Mef2.PR (Vanderploeg et al., 2012, Vanderploeg et al., 2012)
Comments	Expression of scb[Scer\UAS.cVa] under the control of Scer\GAL4[Mef2.PR] partially restores lumen formation between cardioblasts in the heart region of scb[2]/scb[2] embryos. (Vanderploeg et al., 2012)
Stocks ( 2 )
Bloomington	3098 cn¹ scb² bw¹ sp¹/CyO
Kyoto	106839 cn¹ scb² bw¹ sp¹/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	scab^IIG (Arora et al., 1995) scb² (Vanderploeg et al., 2012, Tsai et al., 2008, Christensen et al., 2007.10.29, Wada et al., 2007) scb^IIG (Brown et al., 2000, Martin-Bermudo et al., 1999, Tearle and Nusslein-Volhard, 1987, )
Name Synonym
Secondary FlyBase IDs

References ( 16 )
Research paper	Vanderploeg et al., 2012, BMC Dev. Biol. 12: 8 Integrins are required for cardioblast polarisation in Drosophila. [FBrf0217831] Tsai et al., 2008, Neural Dev. 3: 26 Fak56 functions downstream of integrin alphaPS3betanu and suppresses MAPK activation in neuromuscular junction growth. [FBrf0206161] Wada et al., 2007, Dev. Biol. 301(2): 340--349 Specialized extraembryonic cells connect embryonic and extraembryonic epidermis in response to Dpp during dorsal closure in Drosophila. [FBrf0193483] Brumby et al., 2004, Genetics 168(1): 227--251 A genetic screen for dominant modifiers of a cyclin E hypomorphic mutation identifies novel regulators of S-phase entry in Drosophila. [FBrf0180291] Read et al., 2004, Mol. Cell. Biol. 24(15): 6676--6689 Drosophila C-terminal Src kinase negatively regulates organ growth and cell proliferation through inhibition of the Src, Jun N-terminal kinase, and STAT pathways. [FBrf0179404] Araujo et al., 2003, Development 130(16): 3851--3864 Integrins modulate Sog activity in the Drosophila wing. [FBrf0160375] Bradley et al., 2003, Dev. Biol. 257(2): 249--262 Posterior migration of the salivary gland requires an intact visceral mesoderm and integrin function. [FBrf0158855] Schock and Perrimon, 2003, Genes Dev. 17(5): 597--602 Retraction of the Drosophila germ band requires cell-matrix interaction. [FBrf0157157] Stevens and Jacobs, 2002, J. Neurosci. 22(11): 4448--4455 Integrins regulate responsiveness to slit repellent signals. [FBrf0149077] Grevengoed et al., 2001, J. Cell Biol. 155(7): 1185--1198 Abelson kinase regulates epithelial morphogenesis in Drosophila. [FBrf0141596] Martin-Bermudo et al., 1999, Development 126(22): 5161--5169 Migration of the Drosophila primordial midgut cells requires coordination of diverse PS integrin functions. [FBrf0111966] Stark et al., 1997, Development 124(22): 4583--4594 A novel alpha integrin subunit associates with betaPS and functions in tissue morphogenesis and movement during Drosophila development. [FBrf0099731] Arora et al., 1995, Cell 81(5): 781--790 The Drosophila schnurri gene acts in the Dpp/TGF signaling pathway and encodes a transcription factor homologous to the human MBP family. [FBrf0081322]
Review	Brown et al., 2000, Dev. Biol. 223(1): 1--16 Integrins as mediators of morphogenesis in Drosophila. [FBrf0128416]
Personal communication to FlyBase	Christensen et al., 2007.10.29, Isolation and characterization of Df(2R)BSC330. Isolation and characterization of Df(2R)BSC330. [FBrf0200239]
Stock list	Tearle and Nusslein-Volhard, 1987, D. I. S. 66: 209--269 Tubingen mutants and stock list. [FBrf0045941]

Allele Dmel\scb2

Allele Dmel\scb²