Allele Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}

General Information
Symbol	Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}	Species	a. artificial
Name		FlyBase ID	FBal0127243
Feature type	allele	Associated gene	Zzzz\CAG

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Steffan et al., 2001) in vitro construct - regulatory fusion (Steffan et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS sequences drive expression of 48 Glutamines. This is tagged with T:Hsap\MYC and T:Zzzz\FLAG. (Steffan et al., 2001)
Carried in construct	P{UAS-Q48.myc/flag} (Kazantsev et al., 2002, Steffan et al., 2001, Apostol et al., 2003, Scappini et al., 2007, Lievens et al., 2005)
Tagged with	T:Hsap\MYC (Steffan et al., 2001, Kazantsev et al., 2002, Apostol et al., 2003) T:Zzzz\FLAG (Steffan et al., 2001, Kazantsev et al., 2002, Apostol et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	hypoactive \| adult stage, with Scer\GAL4^Eaat1.PR (Lievens et al., 2005) lethal \| partially, with Scer\GAL4^elav.PLu (Kazantsev et al., 2002, Steffan et al., 2001) neurophysiology defective, with Scer\GAL4^GMR.PF (Scappini et al., 2007) short lived, with Scer\GAL4^Eaat1.PR (Lievens et al., 2005)
Phenotype Manifest In
	eye photoreceptor cell & neuron, with Scer\GAL4^elav.PLu (Kazantsev et al., 2002) photoreceptor, with Scer\GAL4^GMR.PF (Scappini et al., 2007) rhabdomere, with Scer\GAL4^elav.PLu (Steffan et al., 2001)
Detailed Description
	Statement Reference Expression of Zzzz\CAG[Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] in photoreceptors under the control of Scer\GAL4[GMR.PF] results in electroretinogram depolarisation amplitudes that are 55% lower than controls. (Scappini et al., 2007) Flies expressing Zzzz\CAG[Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] under the control of Scer\GAL4[Eaat1.PR] have a shortened lifespan compared to wild type and are found to be lethargic a few days before dying. (Lievens et al., 2005) Expression of Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PLu results in loss of 1-2 photoreceptor neurons per ommatidium and some gaps in the eye tissue. (Kazantsev et al., 2002) Expression of Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} driven by Scer\GAL4^elav.PLu leads to degeneration of rhabdomeres and partial lethality. Suberoylanilide hydroxamic acid (SAHA) suppresses the lethality and reduces the level of degeneration seen in the eye to wild-type levels. (Steffan et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has neurophysiology defective phenotype, enhanceable by Dap160^Scer\UAS.cKa, Scer\GAL4^GMR.PF (Scappini et al., 2007)
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has neurophysiology defective phenotype, non-enhanceable by Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}, Scer\GAL4^GMR.PF (Scappini et al., 2007)
Suppressed by
Statement Reference Scer\GAL4^elav.PLu, Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has partially lethal - majority die phenotype, suppressible \| partially by Hsap\HTT^{Su3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC}, Scer\GAL4^elav.PLu (Kazantsev et al., 2002)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has photoreceptor phenotype, enhanceable by Dap160^Scer\UAS.cKa, Scer\GAL4^GMR.PF (Scappini et al., 2007)
NOT Enhanced by
Statement Reference Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has photoreceptor phenotype, non-enhanceable by Mmus\Cd8a^{Scer\UAS.T:Avic\GFP}, Scer\GAL4^GMR.PF (Scappini et al., 2007)
Suppressed by
Statement Reference Scer\GAL4^elav.PLu, Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} has eye photoreceptor cell & neuron phenotype, suppressible by Hsap\HTT^{Su3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC}, Scer\GAL4^elav.PLu (Kazantsev et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Mmus\Cd8a[Scer\UAS.T:Avic\GFP] does not enhance the reduction in electroretinogram depolarisation amplitude seen when Zzzz\CAG[Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] is expressed in photoreceptors under the control of Scer\GAL4[GMR.PF]. Co-expression of Dap160[Scer\UAS.cKa] enhances the reduction in electroretinogram depolarisation amplitude seen when Zzzz\CAG[Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG] is expressed in photoreceptors under the control of Scer\GAL4[GMR.PF]. (Scappini et al., 2007) Co-expression of Hsap\HD^{Su3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC} significantly suppresses the loss of photoreceptor neurons seen in flies expressing Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PLu. (Kazantsev et al., 2002)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Scappini et al., 2007, Hum. Mol. Genet. 16(15): 1862--1871 Intersectin enhances huntingtin aggregation and neurodegeneration through activation of c-Jun-NH2-terminal kinase. [FBrf0201221] Lievens et al., 2005, Hum. Mol. Genet. 14(5): 713--724 Expanded polyglutamine peptides disrupt EGF receptor signaling and glutamate transporter expression in Drosophila. [FBrf0184022] Apostol et al., 2003, Proc. Natl. Acad. Sci. U.S.A. 100(10): 5950--5955 A cell-based assay for aggregation inhibitors as therapeutics of polyglutamine-repeat disease and validation in Drosophila. [FBrf0159306] Kazantsev et al., 2002, Nat. Genet. 30(4): 367--376 A bivalent Huntingtin binding peptide suppresses polyglutamine aggregation and pathogenesis in Drosophila. [FBrf0147171] Steffan et al., 2001, Nature 413(6857): 739--743 Histone deacetylase inhibitors arrest polyglutamine-dependent neurodegeneration in Drosophila. [FBrf0139769]

Allele Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG

Allele Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}