FB2025_01 , released February 20, 2025
Allele: Dmel\shotLA-Δrod1.UAS.GFP
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General Information
Symbol
Dmel\shotLA-Δrod1.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0137091
Feature type
allele
Associated gene
Dmel\shot
Associated Insertion(s)
Carried in Construct
P{UAS-shot.L(A)-Δrod1-GFP}
Key Links
Transgenic product class
inframe_deletion
(Lee and Kolodziej, 2002)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Lee and Kolodziej, 2002)
Mutagen
in vitro construct
(Lee and Kolodziej, 2002)
Progenitor genotype
shotLA.UAS.GFP
(Lee and Kolodziej, 2002)
Carried in construct
P{UAS-shot.L(A)-Δrod1-GFP}
Cytology
Description

Deletion of sequences encoding shot amino acids 1205 to 4599 (coordinates according to accession number AAF24343). This removes approximately 75% of the central domain, including all the spectrin repeats.

(Lee and Kolodziej, 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
shot
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of shotLA-Δrod1.Scer\UAS.T:Avic\GFP in embryonic motorneurons or tendon cells in a wild-type background does not engender any obvious dominant negative effects.

      (Bottenberg et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      shotLA-Δrod1.UAS.GFP/Scer\GAL4VP16.mat.αTub67C rescues shotGL01286

      (Lu et al., 2021)

      Scer\GAL4elav.PU/shotLA-Δrod1.UAS.GFP rescues shotVV/shot3

      (Valakh et al., 2013)

      Scer\GAL4insc-Mz1407/shotLA-Δrod1.UAS.GFP rescues shot3

      (Lee and Kolodziej, 2002)
      Partially rescues

      Scer\GAL4unspecified/shotLA-Δrod1.UAS.GFP partially rescues shotunspecified

      (Bottenberg et al., 2009)

      Scer\GAL4sr-md710/shotLA-Δrod1.UAS.GFP partially rescues shotunspecified

      (Bottenberg et al., 2009)
      Fails to rescue

      Scer\GAL4how-24B/shotLA-Δrod1.UAS.GFP fails to rescue shotVV/shot3

      (Valakh et al., 2013)
      Comments

      Expression of shotLA-Δrod1.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4elav.PU rescues the increase in bouton number seen at the neuromuscular junction in shot3/shotVV third instar larvae.

      (Valakh et al., 2013)

      Dendrites extend normally in shotunspecified mutant embryos expressing shotLA-Δrod1.Scer\UAS.T:Avic\GFP in motorneurons, but they appear less branched compared to wild-type.

      Scer\GAL4sr-md710 driven expression of shotLA-Δrod1.Scer\UAS.T:Avic\GFP prevents muscle detachment in shotunspecified mutants, but 75% of tendon cells are abnormally elongated.

      (Bottenberg et al., 2009)

      Expression of shotLA-Δrod1.Scer\UAS.T:Avic\GFP under the control of Scer\GAL41407 rescues the motor and sensory axon extension defects seen in shot3 mutants.

      (Lee and Kolodziej, 2002)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      shotLA-Δrod1.Scer\UAS.T:Avic\GFP
      shotLA-Δrod1.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (4)