FlyBase Allele Report: Dmel\sals[f07849]

Primary muscle cultures derived from myoblasts dissociated from homozygous mutant embryos have a reduced sarcomere length and myofibril width, but normal myofibril length compared to control cultures.

Stage 16 homozygous embryos have normal overall muscle morphology, and the number of nuclei in mutant muscles is comparable to that in wild type.

Muscles in late stage 17 mutant embryos have myofibrils with numerous short sarcomeres (they show an approximately 27% reduction in length compared to wild type). In addition, the mutant myofibrils are thin and split and are distributed along the lateral side of muscles. The thin filaments are significantly shorter in length than normal. The nuclei of the mutant muscles are localised more towards the muscle ends (wild-type muscles have even distributed nuclei).

The muscles of mutant larvae at 28-30 hours after egg laying contain many more sarcomeres than those of control larvae, but they are much shorter in length longitudinally compared to controls. Some of the mutant muscles show a complete disruption of myofibril morphology.

Peristaltic movement is detectable in later stage 17 mutant embryos, however, the mutant embryos have less frequent backward peristalsis than controls, and the vigour of their contraction is much reduced. At 28-30 hours after egg laying (the expected hatch time), the mutants show weak movement. They eventually die in the egg case within a day. If mutant animals are manually removed from the egg case, they are flaccid and immobile.

(Bai et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sals^f07849 is an enhancer of lethal | embryonic stage phenotype of Scer\GAL4^Mef2.PR, tmod^UAS.cBa

(Bai et al., 2007)

Phenotype Manifest In

Enhancer of

Statement

Reference

sals^f07849 is an enhancer of striated muscle thin filament phenotype of Scer\GAL4^Mef2.PR, tmod^UAS.cBa

(Bai et al., 2007)

NOT Enhancer of

Statement

Reference

sals^f07849/sals[+] is a non-enhancer of indirect flight muscle cell phenotype of DAAM^Ex1

(Molnár et al., 2014)

NOT Suppressor of

Statement

Reference

sals^f07849/sals[+] is a non-suppressor of indirect flight muscle cell phenotype of DAAM^Ex1

(Molnár et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The mild indirect muscle phenotype seen in DAAM^Ex1 mutants is not altered by sals^f07849/+.

(Molnár et al., 2014)

sals^f07849 enhances the lethality cause by expression of tmod^Scer\UAS.cBa under the control of Scer\GAL4^Mef2.PR, such that a greater fraction of the animals die as embryos.

The reduction in thin filament length seen in primary cultured muscle cells isolated from embryos expressing tmod^Scer\UAS.cBa under the control of Scer\GAL4^Mef2.PR is increased if the animals are also carrying sals^f07849.

(Bai et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sals^f07849 is rescued by sals^{ΔProR.UAS.Tag:FLAG}/Scer\GAL4^Mef2.PU

(Farkas et al., 2024)

sals^f07849 is rescued by sals^{ProR.WH2.Cterm.UAS.Tag:FLAG}/Scer\GAL4^Mef2.PU

(Farkas et al., 2024)

sals^f07849 is rescued by sals^UAS.Tag:FLAG/Scer\GAL4^Mef2.PU

(Farkas et al., 2024)