FB2025_01 , released February 20, 2025
Allele: Dmel\Fsnf06595
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General Information
Symbol
Dmel\Fsnf06595
Species
D. melanogaster
Name
FlyBase ID
FBal0185593
Feature type
allele
Associated gene
Dmel\Fsn
Associated Insertion(s)
PBac{WH}Fsnf06595
Carried in Construct
Also Known As
f06595
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kugler et al., 2010)

hypomorphic allele - genetic evidence

(Wu et al., 2007)
Mutagen
piggyBac activity
(Gene Disruption Project members and Exelixis, 2005)
Progenitor genotype
Associated Insertion(s)
PBac{WH}Fsnf06595
Cytology
Description

Insertion in the second exon, 121bp upstream of the start codon.

(Wu et al., 2007)
Allele components
Component
Use(s)
Inserted element
PBac{WH}
Regulatory region(s)
UAS
Also carries
FRT
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos of the maternal genotype Fsnf06595/Df(2R)Exel7124 have nearly wild-type viability.

      Introducing Fsnf06595/+ or homozygous Fsnf06595 into the maternal Scer\GAL4nos.UTR.T:Hsim\VP16>FsnScer\UAS.T:Ivir\HA1 overexpression background results in some loss of primordial germ cells compared with wild-type.

      Embryos of homozygous maternal Fsnf06595 do not form extra primordial germ cells compared to wild-type.

      (Kugler et al., 2010)

      The neuromuscular junction (NMJ) of homozygous and Fsnf06595/Df(2R)Exel7124 larvae shows dramatic synaptic terminal overgrowth.

      Fsnf06595/Df(2R)Exel7124 mutant larvae show an almost four-fold increase in both bouton number and number of branch points compared to wild type (assayed at the NMJ of the MN4-Ib motorneuron onto muscle 4). The synaptic span (length of type I synapse divided by the length of muscle) is also increased from 18% in wild type to 30% in the Fsnf06595/Df(2R)Exel7124 mutant larvae.

      Homozygous larvae show an increase in bouton number, branch point number and synaptic span at the NMJ.

      Fsnf06595/Df(2R)Exel7124 mutant larvae show a reduction in evoked release at the neuromuscular junction; there is a 70% reduction in the excitatory junctional potential (EJP) amplitude compared to wild type, due to a 66% decrease in quantal content. Quantal size (mEJP amplitude) is unchanged in the mutant larvae.

      (Wu et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Enhancer of
      Statement
      Reference

      Fsnf06595 is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of hiwND51

      (Wu et al., 2007)
      Phenotype Manifest In
      Suppressed by
      Enhancer of
      Statement
      Reference

      Fsnf06595 is an enhancer of embryonic/larval neuromuscular junction phenotype of hiwND51

      (Wu et al., 2007)

      Fsnf06595 is an enhancer of NMJ bouton | increased number phenotype of hiwND51

      (Wu et al., 2007)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      hiwND51 Fsnf06595 double mutant larvae have dramatically overgrown synapses at the neuromuscular junction, with a significant increase in the number of boutons compared to the hiwND51 single mutant and an enhancement of synaptic branching.

      The synaptic terminal overgrowth phenotype seen at the neuromuscular junction is not significantly different in hiwND9 Fsnf06595 double mutant larvae compared to hiwND9 single mutant larvae.

      The synaptic overgrowth phenotypes seen at the neuromuscular junction in Fsnf06595/Df(2R)Exel7124 larvae (increased bouton number, increased number of branch points and increased synaptic span) are completely suppressed by wnd3.

      (Wu et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG4643f06595
      (Kugler and Lasko, 2007)
      Fsnf06595
      (Tian et al., 2011, Kugler et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)