FlyBase Allele Report: Dmel\Rich[1]

General Information

Symbol

Dmel\Rich¹

Species

D. melanogaster

Name

FlyBase ID

FBal0263635

Feature type

allele

Associated gene

Dmel\Rich

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tong et al., 2011)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?380term.

(Tong et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone

(Tong et al., 2011)

abnormal neurophysiology | somatic clone

(Tong et al., 2011)

lethal | recessive

(Tong et al., 2011)

Phenotype Manifest In

optic cartridge | somatic clone

(Tong et al., 2011)

photoreceptor cell R7 | cell autonomous | somatic clone

(Tong et al., 2011)

photoreceptor cell R7 | somatic clone

(Tong et al., 2011)

Detailed Description

Statement

Reference

Rich¹ eye clones made using the 'eyFLP' system display abnormally small "on" and "off" transients in electroretinograms. However, the amplitude of depolarizations in the mutant is fairly normal. Mutant epithelial glial cells are thinner than in wild type, and the cartridges contain a highly variable number of photoreceptor cell terminals ranging from 3 to 8. However, in these missorted cartridges, active zone integrity, vesicle density and capitate projections are normal.

chp staining of the medulla of Rich¹ eye clones (made using the 'eyFLP' system) reveals a few 'gaps' as if some R7 terminals are missing.

About 20% of all R7 cell terminals fail to reach their target layer M6, but rather target the M3 layer in adult brains containing Rich¹ clones made using the 'eyFLP' system. In contrast, there are no obvious R8 targeting defects.

In the medulla, 74% of Rich¹ mutant R7 cells (induced specifically in photoreceptor cells using Scer\FLP1^ey.3.5.hs and MARCM) fail to target to the M6 layers.

62.5% of single Rich¹ mutant R7 cells (induced specifically in photoreceptor cells using Scer\FLP1^ey.3.5.hs and MARCM) surrounded by wild type R7 and R8 cells fail to target to the M6 layer, indicating a cell-autonomous effect.

Animals containing Rich¹ mutant eye clones induced specifically in photoreceptor cells using Scer\FLP1^ey.3.5.hs display improper cartridge organization in the lamina.

(Tong et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Rich¹ has abnormal neuroanatomy | somatic clone phenotype, non-enhanceable by Rab6[+]/Rab6^D23D

(Tong et al., 2011)

Rich¹ has abnormal neuroanatomy | somatic clone phenotype, non-enhanceable by Rab6^T26N.UAS.YFP/Scer\GAL4^Act5C.PP

(Tong et al., 2011)

Rich¹ has abnormal neuroanatomy | somatic clone phenotype, non-enhanceable by CadN[+]/CadN⁴⁰⁵

(Tong et al., 2011)

Suppressed by

Statement

Reference

Rich¹ has abnormal neuroanatomy | somatic clone phenotype, suppressible by Rab6^{Q71L.UASp.YFP}/Scer\GAL4^Act5C.PP

(Tong et al., 2011)

NOT suppressed by

Statement

Reference

Rich¹ has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by Rab6^T26N.UAS.YFP/Scer\GAL4^Act5C.PP

(Tong et al., 2011)

Other

Statement

Reference

CadN^Δ14, Rich¹ has abnormal neuroanatomy | somatic clone phenotype

(Tong et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Rich¹ has photoreceptor cell R7 | somatic clone phenotype, non-enhanceable by Rab6[+]/Rab6^D23D

(Tong et al., 2011)

Rich¹ has photoreceptor cell R7 | somatic clone phenotype, non-enhanceable by Rab6^T26N.UAS.YFP/Scer\GAL4^Act5C.PP

(Tong et al., 2011)

Rich¹ has photoreceptor cell R7 | somatic clone phenotype, non-enhanceable by CadN[+]/CadN⁴⁰⁵

(Tong et al., 2011)

Suppressed by

Statement

Reference

Rich¹ has photoreceptor cell R7 | somatic clone phenotype, suppressible by Rab6^{Q71L.UASp.YFP}/Scer\GAL4^Act5C.PP

(Tong et al., 2011)

NOT suppressed by

Statement

Reference

Rich¹ has photoreceptor cell R7 | somatic clone phenotype, non-suppressible by Rab6^T26N.UAS.YFP/Scer\GAL4^Act5C.PP

(Tong et al., 2011)

Other

Statement

Reference

CadN^Δ14, Rich¹ has photoreceptor cell R7 | somatic clone phenotype

(Tong et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Rab6^D23D/+ does not enhance the R7 targeting phenotype seen in Rich¹ eye clones made using the 'eyFLP' system.

Overexpression of Rab6^{Q71L.Scer\UAS.P\T.T:Avic\GFP-YFP} in Rich¹ eye clones made using the 'MARCM' system greatly suppresses the R7 targeting defects caused by Rich¹.

Overexpression of Rab6^{T26N.Scer\UAS.T:Avic\GFP-YFP} in Rich¹ eye clones made using the 'MARCM' system neither suppresses nor enhances the R7 targeting defects caused by Rich¹.

CadN⁴⁰⁵/+ does not enhance the R7 targeting phenotype seen in Rich¹ eye clones made using the 'eyFLP' system.

CadN^Δ14, Rich¹ double mutant eye clones (made using the 'eyFLP' system) have R7 targeting phenotypes similar to CadN^Δ14 single mutants.

(Tong et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rich¹ is rescued by Rich^+t7.5

(Tong et al., 2011)

Rich¹ is rescued by Rich^Tag:HA

(Tong et al., 2011)

Comments

Rich^+t7.5 rescues the homozygous lethality and the targeting phenotypes seen in Rich¹ eye clones.

Rich^T:Ivir\HA1 rescues the homozygous lethality and the targeting phenotypes seen in Rich¹ eye clones.

(Tong et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51605

w^*; Rich¹ P{neoFRT}80B/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹

Notes on Origin

Discoverer

Comments

Molecular and phenotypic data suggest that Rich² is a weaker allele than Rich¹.

(Tong et al., 2011)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

3L61

(Tong et al., 2011)

Rich¹

(Iwanami et al., 2016)

rich¹

(Tong et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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