FlyBase Allele Report: Dmel\GstO2[A.UAS]

General Information

Symbol

Dmel\GstO2^A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0267936

Feature type

allele

Associated gene

Dmel\GstO2

Associated Insertion(s)

Carried in Construct

P{UAS-GstO2.A}

Key Links

Transgenic product class

wild_type

(Kim et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kim et al., 2012)

Mutagen

in vitro construct

(Kim et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-GstO2.A}

Cytology

Description

UASt regulatory sequences drive expression of GstO2-A coding sequences.

(Kim et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

GstO2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Pink1^B9

(Kim and Yim, 2013)

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^UAS.cEa

(Cha et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

GstO2^A.UAS/Scer\GAL4^Mef2.PU is a suppressor | partially of visible phenotype of Pink1^B9

(Kim and Yim, 2013)

GstO2^A.UAS/Scer\GAL4^Mef2.PU is a suppressor | partially of increased cell death phenotype of Pink1^B9

(Kim and Yim, 2013)

Scer\GAL4^ple.PF/GstO2^A.UAS is a suppressor | partially of abnormal neuroanatomy | adult stage | progressive phenotype of Pink1^B9

(Kim and Yim, 2013)

Scer\GAL4^ple.PF/GstO2^A.UAS is a suppressor of abnormal neuroanatomy phenotype of park¹

(Kim et al., 2012)

NOT Suppressor of

Statement

Reference

GstO2^A.UAS/Scer\GAL4^phtm.PO is a non-suppressor of lethal - all die during embryonic stage | recessive phenotype of GstE14^KO

(Enya et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

GstO2^A.UAS/Scer\GAL4^Mef2.PU is a suppressor | partially of adult thorax phenotype of Pink1^B9

(Kim and Yim, 2013)

GstO2^A.UAS/Scer\GAL4^Mef2.PU is a suppressor | partially of flight muscle cell phenotype of Pink1^B9

(Kim and Yim, 2013)

Scer\GAL4^ple.PF/GstO2^A.UAS is a suppressor | partially of dopaminergic PPL1 neuron | progressive phenotype of Pink1^B9

(Kim and Yim, 2013)

Scer\GAL4^ple.PF/GstO2^A.UAS is a suppressor of dopaminergic PPL1 neuron phenotype of park¹

(Kim et al., 2012)

Scer\GAL4^how-24B/GstO2^A.UAS is a suppressor of wing phenotype of park¹

(Kim et al., 2012)

Scer\GAL4^how-24B/GstO2^A.UAS is a suppressor of adult thorax phenotype of park¹

(Kim et al., 2012)

GstO2^A.UAS/Scer\GAL4^αTub84B.PL is a suppressor of indirect flight muscle cell phenotype of park¹

(Kim et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of GstO2^A.Scer\UAS under the control of Scer\GAL4^phm.PO does not rescue the embryonic lethality associated with homozygous GstE14^KO mutants.

(Enya et al., 2014)

Expression of GstO2^A.Scer\UAS under the control of Scer\GAL4^Mef2.PU significantly decreases the penetrance of the collapsed thorax phenotype seen in Pink1^B9 adults. The increased cell death seen in the flight muscles of 3 day old Pink1^B9 adults is also suppressed.

The loss of dopaminergic neurons in the PPL1 cluster of the brain which is seen in 20 day old Pink1^B9 flies is significantly suppressed by expression of GstO2^A.Scer\UAS under the control of Scer\GAL4^ple.PF.

(Kim and Yim, 2013)

Expression of GstO2^A.Scer\UAS using the muscle-specific driver Scer\GAL4^how-24B significantly suppresses both the thorax and downturned wing phenotypes found in park¹ mutants.

Overexpression of GstO2^A.Scer\UAS under the control of Scer\GAL4^αTub84B.PL, a ubiquitous driver, suppresses the degeneration of IFMs in park¹ mutants. Overexpression results in regular and compact muscle tissues in the dorsal longitudinal IFMs, which are similar to those of wild-type flies except for occasional vacuoles. Overexpression of GstO2^A.Scer\UAS appears to prevent degeneration of the IFMs by blocking the activation of the JNK pathway and apoptosis in park¹ mutants.

Overexpression of GstO2^A.Scer\UAS under the control of Scer\GAL4^ple.PF results in significant restoration of the lost DA neurons in 20-day-old park¹ mutant flies.

(Kim et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

GstO2^A.UAS/Scer\GAL4^αTub84B.PL rescues GstO2¹

(Kim et al., 2012)

Comments

Paraquat sensitivity in GstO2¹ mutants is rescued through Scer\GAL4^αTub84B.PL-driven expression of GstO2^A.Scer\UAS.

(Kim et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG6673^A.Scer\UAS

GstO1^A.Scer\UAS

GstO2^A.Scer\UAS

GstO2^A.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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