FB2025_01 , released February 20, 2025
Allele: Dmel\Cad99CEXTRA.UAS.Tag:HA
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General Information
Symbol
Dmel\Cad99CEXTRA.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0270006
Feature type
allele
Associated gene
Dmel\Cad99C
Associated Insertion(s)
Carried in Construct
P{UAS-Cad99C.EXTRA-HA}
Key Links
Transgenic product class
inframe_deletion
(Schlichting et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Schlichting et al., 2006)
Mutagen
in vitro construct
(Schlichting et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Cad99C.EXTRA-HA}
Cytology
Description

UASt regulatory sequences drive expression of amino acids 1-1419 of Cad99C (the extracellular and transmembrane domains) which are tagged at the C-terminal end with a triple Tag:HA tag.

(Schlichting et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Cad99C
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Cad99CEXTRA.Scer\UAS.T:Ivir\HA1 in the salivary gland under the control of Scer\GAL4fkh.PH results in apical salivary gland membrane expansion. These mutants exhibit an increase in microvilli-like structures and causes apical membranes to protrude into the lateral domain. Mutant salivary glands display stretched adherens junctions and wider apical domains in each cell compared to wild-type, consistent with an increase in apical domain size.

      Salivary gland cells expressing Cad99CEXTRA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4fkh.PH reveal rounded basal surfaces and wider spaces between neighboring cells, indicating a loss of cell-cell contact nearly everywhere other than the septate junction.

      (Chung and Andrew, 2014)

      Follicle cell clones expressing Cad99CEXTRA.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PP have abnormal bundles of microvilli.

      (Schlichting et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      Statement
      Reference

      Cad99CEXTRA.UAS.Tag:HA, Scer\GAL4fkh.PH has abnormal cell adhesion phenotype, non-suppressible by sas15

      (Chung and Andrew, 2014)
      Phenotype Manifest In
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A sas15 mutant background does not affect the Cad99CEXTRA.Scer\UAS.T:Ivir\HA1 overexpression phenotype.

      (Chung and Andrew, 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Cad99CEXTRA.Scer\UAS.T:Ivir\HA1
      Cad99CEXTRA.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)