Reference Report

Reference
Citation	Schlichting, K., Wilsch-Brauninger, M., Demontis, F., Dahmann, C. (2006). Cadherin Cad99C is required for normal microvilli morphology in Drosophila follicle cells. J. Cell Sci. 119(6): 1184--1195. (Export to RIS)
FlyBase ID	FBrf0190963
Publication Type	Research paper
PubMed ID	16507588
PubMed Abstract	Microvilli are actin-filled membranous extensions common to epithelial cells. Several proteins have been identified that localize to microvilli. However, most of these proteins are dispensable for the normal morphogenesis of microvilli. Here, we show by immunoelectron microscopy that the non-classical cadherin Cad99C localizes to microvilli of Drosophila ovarian follicle cells. Loss of Cad99C function leads to disorganized and abnormal follicle cell microvilli. Conversely, overexpression of Cad99C in follicle cells results in large bundles of microvilli. Furthermore, altered microvilli morphology correlates with defects in the assembly of the vitelline membrane, an extracellular layer secreted by follicle cells that is part of the eggshell. Finally, we provide evidence that Cad99C is the homolog of vertebrate protocadherin 15. Mutations in the gene encoding protocadherin 15 lead to the disorganization of stereocilia, which are microvilli-derived extensions of cochlear hair cells, and deafness (Usher syndrome type 1F). Our data suggest an essential role for Cad99C in microvilli morphogenesis that is important for follicle cell function. Furthermore, these results indicate that insects and vertebrates use related cadherins to organize microvilli-like cellular extensions.
DOI	10.1242/jcs.02831
Related Publication(s)
Erratum	Cadherin Cad99C is required for normal microvilli morphology in Drosophila follicle cells (vol 119, pg 1184, 2006). Schlichting et al., 2006, J. Cell Sci. 119(6): 1197 [FBrf0194796]
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Alleles (13)
	Cad99C^51C Cad99C^57A Cad99C^120B Cad99C^248A Cad99C^{EXTRA.Scer\UAS.T:Ivir\HA1} Cad99C^{INTRA.Scer\UAS.T:Ivir\HA1} Cad99C^{Scer\UAS.T:Avic\GFP-EGFP} Cad99C^{Scer\UAS.T:Ivir\HA1} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Act5C.PP Scer\GAL4^CY2 Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} T:Avic\GFP^EGFP
Constructs (6)
	P{GAL4-Act5C(FRT.CD2).P} P{UAS-Cad99C.EXTRA-HA} P{UAS-Cad99C.GFP} P{UAS-Cad99C.HA} P{UAS-Cad99C.INTRA-HA} P{UAS-mCD8::GFP.L}
Genes (9)
	Cad99C Cp18 Cp36 Mmus\Cd8a Scer\GAL4 shg T:Ivir\HA1 Vm26Aa Vm26Ab
Insertions (3)
	P{EP}Cad99C^GE21034 P{EP}Cad99C^GE23478 P{GawB}CY2
Natural transposons (1)
	P-element