FlyBase Allele Report: Dmel\LamC[UAS.cGa]

General Information

Symbol

Dmel\LamC^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Gurudatta

FlyBase ID

FBal0283352

Feature type

allele

Associated gene

Dmel\LamC

Associated Insertion(s)

Carried in Construct

P{UAS-LamC.G}

Also Known As

UAS-lamC

Key Links

Transgenic product class

cDNA

(Gurudatta et al., 2010)

wild_type

(Gurudatta et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Gurudatta et al., 2010)

wild_type

(Gurudatta et al., 2010)

Mutagen

in vitro construct

(Gurudatta et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-LamC.G}

Cytology

Description

UASt regulatory sequences drive expression of a full-length LamC cDNA.

(Gurudatta et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

LamC

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | third instar larval stage, with Scer\GAL4^ptc-559.1

(Gurudatta et al., 2010)

lethal - all die before end of first instar larval stage, with Scer\GAL4^how-24B

(Gurudatta et al., 2010)

lethal - all die before end of pupal stage, with Scer\GAL4^Act5C.PI

(Mallik and Lakhotia, 2011)

majority die during larval stage, with Scer\GAL4^Act5C.PI

(Mallik and Lakhotia, 2011)

some die during pupal stage, with Scer\GAL4^Act5C.PI

(Mallik and Lakhotia, 2011)

Phenotype Manifest In

anterior-posterior compartment boundary of the wing disc | third instar larval stage, with Scer\GAL4^ptc-559.1

(Gurudatta et al., 2010)

indirect flight muscle cell, with Scer\GAL4^pnr-MD237

(Gurudatta et al., 2010)

longitudinal muscle cell | first instar larval stage, with Scer\GAL4^how-24B

(Gurudatta et al., 2010)

nucleus, with Scer\GAL4^ptc-559.1

(Gurudatta et al., 2010)

nucleus, with Scer\GAL4^{Ubx-lac1-Gal4}

(Gurudatta et al., 2010)

Detailed Description

Statement

Reference

Expression of LamC^UAS.cGa under the control of Scer\GAL4^Act5C.PI results in death of the majority of animals at the larval stage, with the remainder dying shortly after pupation.

(Mallik and Lakhotia, 2011)

Overexpression of LamC^Scer\UAS.cGa under the control of Scer\GAL4^elav.PU does not affect central nervous system development and adult flies are normal.

Scer\GAL4^{Ubx-lac1-Gal4}-mediated LamC^Scer\UAS.cGa expression in the wing disc gives rise to aberrant nuclear morphology.

LamC^Scer\UAS.cGa expression mediated by the early muscle-specific driver Scer\GAL4^how-24B leads to severe defects in the formation of larval longitudinal muscles and, probably as a consequence, lethality at the first instar larval stage.

Scer\GAL4^pnr-MD237-mediated LamC^Scer\UAS.cGa expression leads to the disorganization of dorsal indirect flight muscles in the adult.

Overexpression of LamC^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 leads to extensive apoptotic cell death along the anterior-posterior boundary in wing discs. Compared with surrounding tissue, highly condensed nuclei are observed in the Scer\GAL4^ptc-559.1-domain where LamC^Scer\UAS.cGa is overexpressed.

(Gurudatta et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

LamC^UAS.cGa, Scer\GAL4^Act5C.PI has lethal - all die before end of pupal stage phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS, Scer\GAL4^Act5C.PI

(Mallik and Lakhotia, 2011)

LamC^UAS.cGa, Scer\GAL4^ptc-559.1 has increased cell death | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ptc-559.1

(Gurudatta et al., 2010)

Other

Statement

Reference

LamC^UAS.cGa, Scer\GAL4^Act5C.PI, lncRNA:Hsrω^RNAi.Sym.UAS has visible | pharate adult stage phenotype

(Mallik and Lakhotia, 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

LamC^UAS.cGa, Scer\GAL4^ptc-559.1 has nucleus phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ptc-559.1

(Gurudatta et al., 2010)

LamC^UAS.cGa, Scer\GAL4^ptc-559.1 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ptc-559.1

(Gurudatta et al., 2010)

Other

Statement

Reference

LamC^UAS.cGa, Scer\GAL4^Act5C.PI, lncRNA:Hsrω^RNAi.Sym.UAS has adult head capsule | pharate adult stage phenotype

(Mallik and Lakhotia, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of LamC^UAS.cGa and lncRNA:Hsrω^{dsRNA.Sym.UAS} under the control of Scer\GAL4^Act5C.PI delays the lethality seen with LamC^UAS.cGa/Scer\GAL4^Act5C.PI, so that most die as pupae or pharate adults; the majority of pharates show a severe head capsule defect, with loss of most head structures; the few adults that emerge are all female.

(Mallik and Lakhotia, 2011)

Xenogenetic Interactions

Statement

Reference

The abnormal nuclear morphology and apoptosis caused by the overexpression of LamC^Scer\UAS.cGa under the control of Scer\GAL4^ptc-559.1 is suppressed by BacA\p35^Scer\UAS.cHa.

(Gurudatta et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

LamC^Scer\UAS.cGa

LamC^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Gurudatta

Secondary FlyBase IDs

References (2)

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