FlyBase Allele Report: Dmel\aurB[2A43]

General Information

Symbol

Dmel\aurB^2A43

Species

D. melanogaster

Name

FlyBase ID

FBal0301281

Feature type

allele

Associated gene

Dmel\aurB

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Mathieu et al., 2013)

Progenitor genotype

Cytology

Description

aurB^2A43 mutants exhibit a frame shift followed by a truncation in the kinase activation loop.

(Mathieu et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:10,857,286..10,857,304 [-]

Comment:

Estimated coordinates of a 19 bp deletion that causes a frameshift at amino acid 213 and early translation termination.

(Mathieu et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | third instar larval stage (with aurB¹⁶⁸⁹)

(Gallaud et al., 2022)

lethal

(Mathieu et al., 2013)

Phenotype Manifest In

germarium | germline clone

(Mathieu et al., 2013)

germline cell | germline clone

(Mathieu et al., 2013)

larval neuroblast | third instar larval stage (with aurB¹⁶⁸⁹)

(Gallaud et al., 2022)

nucleus | germline clone

(Mathieu et al., 2013)

nurse cell spectrosome | germline clone

(Mathieu et al., 2013)

Detailed Description

Statement

Reference

Neuroblasts in aurB¹⁶⁸⁹/aurB^2A43 transheterozygous third instar larval brains show increased time in mitosis

(Gallaud et al., 2022)

aurB^2A43 mutant germline clones exhibit defects in early germ-cell development, with the formation of a giant germline stem cell that fills the entire germarium. These mutant cells display highly polyploid nuclei, enlarged spectrosomes and do not come out of the germarium.

aurB^2A43 mutants display highly polyploidy cells and early arrest of oogenesis.

(Mathieu et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

Suppressed by

Statement

Reference

aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

NOT suppressed by

Statement

Reference

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by polo^{T182A.UASp.RR.GFP}/Scer\GAL4^wor.PA

(Gallaud et al., 2022)

aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by polo^UASp.RR.GFP/Scer\GAL4^wor.PA

(Gallaud et al., 2022)

Enhancer of

Statement

Reference

aurB¹⁶⁸⁹/aurB^2A43 is an enhancer of decreased occurrence of cell division | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Suppressor of

Statement

Reference

aurB^2A43/aurB[+] is a suppressor of abnormal cytokinesis | dominant phenotype of shrb^G5

(Matias et al., 2015)

aurB^2A43/aurB[+] is a suppressor | partially of abnormal cell cycle phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

Suppressed by

Statement

Reference

aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

NOT suppressed by

Statement

Reference

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, non-suppressible by polo^{T182A.UASp.RR.GFP}/Scer\GAL4^wor.PA

(Gallaud et al., 2022)

aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, non-suppressible by polo^UASp.RR.GFP/Scer\GAL4^wor.PA

(Gallaud et al., 2022)

Enhancer of

Statement

Reference

aurB¹⁶⁸⁹/aurB^2A43 is an enhancer of larval neuroblast | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Suppressor of

Statement

Reference

aurB^2A43/aurB[+] is a suppressor of egg chamber phenotype of shrb^G5

(Matias et al., 2015)

aurB^2A43/aurB[+] is a suppressor of female germline cell phenotype of shrb^G5

(Matias et al., 2015)

aurB^2A43/aurB[+] is a suppressor of female germline stem cell phenotype of shrb^G5

(Matias et al., 2015)

aurB^2A43/aurB[+] is a suppressor | partially of female germline cyst phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

aurB^2A43/aurB[+] is a suppressor | partially of female fusome phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

aurB^2A43/aurB[+] is a suppressor | partially of egg chamber phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

aurB^2A43/aurB[+] is a suppressor | partially of ring canal phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

aurB^2A43/aurB[+] is a suppressor | partially of nurse cell phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

aurB^2A43/aurB[+] is a suppressor | partially of oocyte phenotype of Det^{S125E.UASp.GFP}, Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The fraction of egg chambers containing 32 cells and the fraction of germaria containing 'stem cysts' in which the female germline stem cells are interconnected to daughter cells is reduced in aurB^2A43/shrb^G5 double heterozygotes compared to shrb^G5 heterozygotes.

(Matias et al., 2015)

A aurB^2A43/+ background partially rescues the extra germline cells phenotype found in females expressing Det^{S125E.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Mathieu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

aurB^L27

(Taniguchi et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

2A43

(Mathieu et al., 2013)

aurB^2A43

(Taniguchi et al., 2018, Matias et al., 2015)

ial^2A43

(Gallaud et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close