FlyBase Allele Report: Dmel\aurB[1689]

General Information

Symbol

Dmel\aurB¹⁶⁸⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0301283

Feature type

allele

Associated gene

Dmel\aurB

Associated Insertion(s)

Carried in Construct

Also Known As

ial¹⁶⁸⁹

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Radford et al., 2012)

Mutagen

ethyl methanesulfonate

(Mathieu et al., 2013)

Progenitor genotype

Cytology

Description

Amino acid replacement: P28S.

(Mathieu et al., 2013, Radford et al., 2012)

Contains an amino acid replacement in the non-conserved N-terminal part of the protein.

(Mathieu et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:10,857,896..10,857,896 [-]

Nucleotide change:

C10857896T

Amino acid change:

P28S | aurB-PA

Reported amino acid change:

P28S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Mathieu et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle

(Mathieu et al., 2013)

abnormal meiotic cell cycle | oogenesis

(Radford et al., 2012)

decreased occurrence of cell division | third instar larval stage (with aurB^2A43)

(Gallaud et al., 2022)

decreased occurrence of cell division | third instar larval stage (with aurB^35.33)

(Gallaud et al., 2022)

viable

(Mathieu et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Neuroblasts in aurB¹⁶⁸⁹/aurB^2A43 and aurB^35.33/aurB¹⁶⁸⁹ transheterozygous third instar larval brains show increased time in mitosis

(Gallaud et al., 2022)

aurB¹⁶⁸⁹ is a hypomorphic mutation, leading to defects in early germ-cell development. Mutant egg chambers consist of 8 germline cells instead of 16 and the oocyte is kinked to only 3 nurse cells by 3 ring canals instead of 4, suggesting that the mutant egg chambers have gone through 3 divisions instead of 4.

aurB¹⁶⁸⁹ mutants exhibit two-cell cysts linked by a thin thread of fusome, indicating that an abscission is about to be completed. These threads of fusome are never seen in wild-type cysts. Eight cell cysts are found in pairs at a frequency three times higher than expected, suggesting that each pair of eight cell cysts is derived from a single precursor.

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ oocytes exhibit only one focus for each pair of centromeres and and mono-orientation of homologous chromosomes (in some cases) while they exhibit bipolar spindles and chromosome movements as compared to controls

(Radford et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

Suppressed by

Statement

Reference

aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

NOT suppressed by

Statement

Reference

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by polo^{T182A.UASp.RR.GFP}/Scer\GAL4^wor.PA

(Gallaud et al., 2022)

aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by polo^UASp.RR.GFP/Scer\GAL4^wor.PA

(Gallaud et al., 2022)

Enhancer of

Statement

Reference

aurB¹⁶⁸⁹/aurB^35.33 is an enhancer of decreased occurrence of cell division | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

aurB¹⁶⁸⁹/aurB^2A43 is an enhancer of decreased occurrence of cell division | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

Suppressed by

Statement

Reference

aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^wor.PA/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurB¹⁶⁸⁹ has female germline cyst phenotype, suppressible by bam[+]/bam^Δ86

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has female fusome phenotype, suppressible by bam[+]/bam^Δ86

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has egg chamber phenotype, suppressible by Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has germline cell phenotype, suppressible by Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has ring canal phenotype, suppressible by Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has nurse cell phenotype, suppressible by Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has female germline cyst phenotype, suppressible by Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has female fusome phenotype, suppressible by Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has egg chamber phenotype, suppressible by bam[+]/bam^Δ86

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has germline cell phenotype, suppressible by bam[+]/bam^Δ86

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has ring canal phenotype, suppressible by bam[+]/bam^Δ86

(Mathieu et al., 2013)

aurB¹⁶⁸⁹ has nurse cell phenotype, suppressible by bam[+]/bam^Δ86

(Mathieu et al., 2013)

NOT suppressed by

Statement

Reference

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, non-suppressible by polo^{T182A.UASp.RR.GFP}/Scer\GAL4^wor.PA

(Gallaud et al., 2022)

aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, non-suppressible by polo^UASp.RR.GFP/Scer\GAL4^wor.PA

(Gallaud et al., 2022)

Enhancer of

Statement

Reference

aurB¹⁶⁸⁹/aurB^35.33 is an enhancer of larval neuroblast | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

aurB¹⁶⁸⁹/aurB^2A43 is an enhancer of larval neuroblast | third instar larval stage phenotype of aurA⁸⁸³⁹

(Gallaud et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Removal of one copy of bam, through a bam^Δ86/+ strongly suppresses the aurB¹⁶⁸⁹ phenotype (from 17% to 4% of eight cell cysts), indicating that the phenotype is caused by the reduction of aurB activity in the cyst and not in the germline stem cell.

Expression of CycB^{5A.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{bam.T:Hsim\VP16} in aurB¹⁶⁸⁹ mutant cysts induces a strong reduction in the number of eight-cell cysts from 14.5% to 3.5% of eight-cell cysts.

(Mathieu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

aurB¹⁶⁸⁹ is rescued by Scer\GAL4^{VP16.nanos.UTR}/aurB^UASp.Tag:HA

(Mathieu et al., 2013)

Comments

Expression of aurB^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the aurB¹⁶⁸⁹ mutant egg chambers that display only 8 cells.

(Mathieu et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

1689

(Mathieu et al., 2013)

aurB¹⁶⁸⁹

(Yang et al., 2022, Das et al., 2016)

ial¹⁶⁸⁹

(Gallaud et al., 2022, Radford et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (5)

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