Reference Report

Reference
Citation	Dolezal, T., Gazi, M., Zurovec, M., Bryant, P.J. (2003). Genetic analysis of the ADGF multigene family by homologous recombination and gene conversion in Drosophila. Genetics 165(2): 653--666. (Export to RIS)
FlyBase ID	FBrf0167663
Publication Type	Research paper
PubMed ID	14573477
PubMed Abstract	Many Drosophila genes exist as members of multigene families and within each family the members can be functionally redundant, making it difficult to identify them by classical mutagenesis techniques based on phenotypic screening. We have addressed this problem in a genetic analysis of a novel family of six adenosine deaminase-related growth factors (ADGFs). We used ends-in targeting to introduce mutations into five of the six ADGF genes, taking advantage of the fact that five of the family members are encoded by a three-gene cluster and a two-gene cluster. We used two targeting constructs to introduce loss-of-function mutations into all five genes, as well as to isolate different combinations of multiple mutations, independent of phenotypic consequences. The results show that (1) it is possible to use ends-in targeting to disrupt gene clusters; (2) gene conversion, which is usually considered a complication in gene targeting, can be used to help recover different mutant combinations in a single screening procedure; (3) the reduction of duplication to a single copy by induction of a double-strand break is better explained by the single-strand annealing mechanism than by simple crossing over between repeats; and (4) loss of function of the most abundantly expressed family member (ADGF-A) leads to disintegration of the fat body and the development of melanotic tumors in mutant larvae.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (17)
	Dp(3;3;3)Berta Dp(3;3;3)Hubert Dp(3;3)Albert Dp(3;3)Amalie Dp(3;3)CD4 Dp(3;3)CD5 Dp(3;3)CD7 Dp(3;3)Drahomira Dp(3;3)Ferda Dp(3;3)Franta Dp(3;3)Gerda Dp(3;3)Hermina Dp(3;3)Karel Dp(3;3)Mojmir Dp(3;3)Rudolf Dp(3;3)Teodor Dp(3;3)Viktor
Alleles (156)
	Adgf-A^Albert.3' Adgf-A^Albert.5' Adgf-A^Am3 Adgf-A^Am7 Adgf-A^Amalie.3' Adgf-A^Amalie.5' Adgf-A^Berta.3' Adgf-A^Berta.5' Adgf-A^Berta.mid Adgf-A^Drahomira.3' Adgf-A^Drahomira.5' Adgf-A^Ferda.3' Adgf-A^Ferda.5' Adgf-A^Fr1 Adgf-A^Fr2 Adgf-A^Fr3 Adgf-A^Fr6 Adgf-A^Fr7 Adgf-A^Fr21 Adgf-A^Fr24 Adgf-A^Franta.3' Adgf-A^Franta.5' Adgf-A^Gerda.3' Adgf-A^Gerda.5' Adgf-A^Hermina.3' Adgf-A^Hermina.5' Adgf-A^HR.cDa Adgf-A^hs.PD Adgf-A^Hubert.3' Adgf-A^Hubert.5' Adgf-A^Hubert.mid Adgf-A^Karel.3' Adgf-A^Karel.5' Adgf-A^Mojmir.3' Adgf-A^Mojmir.5' Adgf-A^Rudolf.3' Adgf-A^Rudolf.5' Adgf-A^{Scer\UAS.T:Hsap\MYC} Adgf-A^Teodor.3' Adgf-A^Teodor.5' Adgf-A^Viktor.3' Adgf-A^Viktor.5' Adgf-B^Albert.3' Adgf-B^Albert.5' Adgf-B^Am3 Adgf-B^Am7 Adgf-B^Amalie.3' Adgf-B^Amalie.5' Adgf-B^Berta.3' Adgf-B^Berta.5' Adgf-B^Berta.mid Adgf-B^Drahomira.3' Adgf-B^Drahomira.5' Adgf-B^Ferda.3' Adgf-B^Ferda.5' Adgf-B^Fr1 Adgf-B^Fr2 Adgf-B^Fr3 Adgf-B^Fr6 Adgf-B^Fr7 Adgf-B^Fr21 Adgf-B^Fr24 Adgf-B^Franta.3' Adgf-B^Franta.5' Adgf-B^Gerda.3' Adgf-B^Gerda.5' Adgf-B^Hermina.3' Adgf-B^Hermina.5' Adgf-B^HR.cDa Adgf-B^Hubert.3' Adgf-B^Hubert.5' Adgf-B^Hubert.mid Adgf-B^Karel.3' Adgf-B^Karel.5' Adgf-B^Mojmir.3' Adgf-B^Mojmir.5' Adgf-B^Rudolf.3' Adgf-B^Rudolf.5' Adgf-B^Teodor.3' Adgf-B^Teodor.5' Adgf-B^Viktor.3' Adgf-B^Viktor.5' Adgf-C^HR2 Adgf-C^HR4.3' Adgf-C^HR4.5' Adgf-C^HR5.3' Adgf-C^HR5.5' Adgf-C^HR7.3' Adgf-C^HR7.5' Adgf-C^HR12 Adgf-C^HR16 Adgf-C^HR17 Adgf-C^HR19 Adgf-C^HR37 Adgf-C^HR46 Adgf-C^HR59 Adgf-C^HR67 Adgf-C^HR.cDa Adgf-D^HR2 Adgf-D^HR4.3' Adgf-D^HR4.5' Adgf-D^HR5.3' Adgf-D^HR5.5' Adgf-D^HR7.3' Adgf-D^HR7.5' Adgf-D^HR12 Adgf-D^HR16 Adgf-D^HR17 Adgf-D^HR19 Adgf-D^HR37 Adgf-D^HR46 Adgf-D^HR59 Adgf-D^HR67 Adgf-D^HR.cDa Msi^Albert.3' Msi^Albert.5' Msi^Am3 Msi^Am7 Msi^Amalie.3' Msi^Amalie.5' Msi^Berta.3' Msi^Berta.5' Msi^Berta.mid Msi^Drahomira.3' Msi^Drahomira.5' Msi^Ferda.3' Msi^Ferda.5' Msi^Fr1 Msi^Fr2 Msi^Fr3 Msi^Fr6 Msi^Fr7 Msi^Fr21 Msi^Fr24 Msi^Franta.3' Msi^Franta.5' Msi^Gerda.3' Msi^Gerda.5' Msi^Hermina.3' Msi^Hermina.5' Msi^HR.cDa Msi^Hubert.3' Msi^Hubert.5' Msi^Hubert.mid Msi^Karel.3' Msi^Karel.5' Msi^Mojmir.3' Msi^Mojmir.5' Msi^Rudolf.3' Msi^Rudolf.5' Msi^Teodor.3' Msi^Teodor.5' Msi^Viktor.3' Msi^Viktor.5' Scer\GAL4^Act5C.PI w^+mW.hs
Constructs (5)
	P{Act5C-GAL4} P{hs-Adgf-A.D} P{TV2.FRT(ADGF-A2^-.-A^-.-B^-.w⁺)} P{TV2.FRT(ADGF-C^-.-D^-.w⁺)} P{UAS-Adgf-A.myc}
Genes (7)
	Adgf-A Adgf-B Adgf-C Adgf-D Msi Scer\GAL4 w
Insertions (7)
	P{TV2.FRT(ADGF-A2^-.-A^-.-B^-.w⁺)}1A P{TV2.FRT(ADGF-A2^-.-A^-.-B^-.w⁺)}3A P{TV2.FRT(ADGF-A2^-.-A^-.-B^-.w⁺)}4 P{TV2.FRT(ADGF-A2^-.-A^-.-B^-.w⁺)}6 P{TV2.FRT(ADGF-A2^-.-A^-.-B^-.w⁺)}9 P{TV2.FRT(ADGF-C^-.-D^-.w⁺)}d3 P{TV2.FRT(ADGF-C^-.-D^-.w⁺)}d11