Dmel\P{lacW}Hsp83^j5C2 Insertion

General Information
Symbol	Dmel\P{lacW}Hsp83^j5C2	Species	D. melanogaster
Name		FlyBase ID	FBti0004973
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, Hsp83	Viability / fertility
Causes allele(s)	Ecol\lacZ^Hsp83-j5C2, Hsp83^j5C2	Stock availability	2 publicly available
LINE ID	l(3)j5C2
Genomic Location
Chromosomal location	3L ( 63B11 )	Sequence location	3L:3,193,242..3,193,242 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:3,193,242..3,193,242 [+] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	63B11 ( inferred by FlyBase from sequence location )
Cytological location (reported)	63B7-63B8 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026323 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Braun et al., 1997, Manfruelli et al., 1999) Hsp83 (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Hsp83-j5C2 (Braun et al., 1997, Manfruelli et al., 1999) Hsp83^j5C2 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Braun et al., 1997)

Lethality References lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference 76% of Hsp83[08445]/Hsp83[j5C2] flies show a loss of locomotor behavioral rhythms under free-running conditions (after entrainment in light-dark cycles). Hsp83[08445]/Hsp83[j5C2] flies show a reduced fraction of flies with rhythmic locomotor activity in light-dark cycles (47% rhythmic) compared to control flies (88%-95% rhythmic). Hsp83[08445]/Hsp83[j5C2] mutants show highly variable locomotor activity patterns, including fully rhythmic, arrhythmic, and complex behavioral profiles, in contrast to wild-type flies, which show little variation among individuals. (Hung et al., 2009) The mutant abdominal segmentation phenotype seen in females carrying nos^ΔBX in a nos^BN/nos^BN background is not affected if the females also carry one copy of Hsp83^j5C2. (Song et al., 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)j5C2 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	12064 w^*; P{lacW}Hsp83^j5C2/TM2
Kyoto	111379 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Hsp83^j5C2 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹
Linkouts
Comments
	Location 3L:3176773-3176774 determined by FlyBase alignment of dbGSS accession AQ026323 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	l(3)j5c2 (Lee and Treisman, 2001) P{lacW}Hsp83^j5C2 (FlyBase, 2005, FlyBase, 1992-) P{lacW}l(3)j5C2 (Manfruelli et al., 1999) P{lacW}l(3)j5C2^j5C2 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{w^+mC=lacW}l(3)j5C2^j5C2 (FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0001188
References ( 14 )
Research paper	Hung et al., 2009, J. Biol. Rhythms 24(3): 183--192 HSP90, a Capacitor of Behavioral Variation. [FBrf0207993] Song et al., 2007, Genetics 176(4): 2213--2222 The molecular chaperone Hsp90 is required for mRNA localization in Drosophila melanogaster embryos. [FBrf0202066] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Lee and Treisman, 2001, Curr. Biol. 11(14): 1147--1152 Sightless has homology to transmembrane acyltransferases and is required to generate active Hedgehog protein. [FBrf0137202] Manfruelli et al., 1999, EMBO J. 18(12): 3380--3391 A mosaic analysis in Drosophila fat body cells of the control of antimicrobial peptide genes by the Rel proteins Dorsal and DIF. [FBrf0110270] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Braun et al., 1997, Genetics 147(2): 623--634 Drosophila immunity: analysis of larval hemocytes by P-element-mediated enhancer trap. [FBrf0098749] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Parks et al., 2004, Nature Genetics 36(3): Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase	Deal et al., 2002.11.12, Isolation and characterization of Df(3L)BSC23. Isolation and characterization of Df(3L)BSC23. [FBrf0151687]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}Hsp83j5C2 Insertion

Dmel\P{lacW}Hsp83^j5C2 Insertion