Dmel\P{lacW}l(2)01528^k13715a Insertion

General Information
Symbol	Dmel\P{lacW}l(2)01528^k13715a	Species	D. melanogaster
Name		FlyBase ID	FBti0008832
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	l(2)01528, Mtp	Viability / fertility
Causes allele(s)	l(2)01528^k13715a	Stock availability	none publicly available
LINE ID	l(2)k13715
Genomic Location
Chromosomal location	2L ( 38F5 )	Sequence location	2L:20,916,731..20,916,839
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)	2 (BDGP Project Members, 1994-1999)
Sequence Location	2L:20,916,731..20,916,839
Orientation
Cytological location (computed by FlyBase)	38F5 ( inferred by FlyBase from sequence location )
Cytological location (reported)	38F1-38F2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AZ575479 (FlyBase, 1996-, Butler et al., 2001)
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	l(2)01528 (Spradling, 1999.12.9, Butler et al., 2001) Mtp (Butler et al., 2001)
Alleles and Phenotypes
Causes alleles	l(2)01528^k13715a (Spradling, 1999.12.9, Butler et al., 2001)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k13715 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line	t (Gene Disruption Project members, 2001-)
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Availability	No longer maintained in the Berkeley P stock collection. (BDGP Project Members, 1994-1999)
Linkouts
Comments
	Line not well characterized (BDGP Project Members, 1994-1999) This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}k13715 (FlyBase, 1992-) P{lacW}k13715a (FlyBase, 1992-) P{lacW}l(2)01528^k13715a (FlyBase, 1996-, Butler et al., 2001, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 10 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Butler et al., 2001, Genetics 158(4): 1597--1614 Map position and expression of the genes in the 38 region of Drosophila. [FBrf0138405] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Spradling, 1999.12.9, Helping FlyBase with P-element paper. Helping FlyBase with P-element paper. [FBrf0125024] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}l(2)01528k13715a Insertion

Dmel\P{lacW}l(2)01528^k13715a Insertion