A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}CRMPEP3238 Insertion

General Information
Symbol Dmel\P{EP}CRMPEP3238 Species D. melanogaster
Name FlyBase ID FBti0011410
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) CRMP Viability / fertility
Causes allele(s) CRMPEP3238 Stock availability 1 publicly available
LINE ID EP(3)3238
Genomic Location
Chromosomal location 3R ( 83B2 ) Sequence location 3R:1,396,842..1,396,842 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0101678 FBti0064954 FBti0041004 FBti0074845 FBti0040111 FBti0131320 FBti0057189 FBti0071017 FBti0039507 FBti0043989 FBti0066238 FBti0011410 FBti0023378 FBti0056742 FBti0074739 FBti0039705 FBti0029341 FBti0112339 FBti0052275 FBti0150237 FBti0125920_2 FBti0125920_1
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:1,396,842..1,396,842 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
83B2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
83B2-83B2 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073846
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
CRMP
(BDGP Project Members, 2000-, Morris et al., 2012)
hide Alleles and Phenotypes
Causes alleles
CRMPEP3238
(Pena-Rangel et al., 2002, Rawls, 2006, Morris et al., 2012)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Under free-running conditions (constant darkness), wild-type and CRMP[EP3238] mutant animals exhibit similar overall activity. Period length is similar for all tested animals, indicating that the length of the circadian clock is not affected by CRMP[EP3238]. Compared with wild-type and heterozygous animals, significantly reduced arrhythmic power values indicating the relative strength of rhythmicity of free-running behaviours are observed among CRMP[EP3238] homozygotes. Some individual CRMP[supK1] homozygotes and CRMP[supK1]/Df(3R)noi-B hemizygotes lack significantly rhythmic behaviours (arrhythmic). Some individual CRMP[EP3238] homozygotes hemizygotes lack significantly rhythmic behaviours (arrhythmic).
(Morris et al., 2012)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(3)3238
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
40953
w1118; P{EP}CRMPEP3238
hide Linkouts
hide Comments
Location 3R:1396842-1396843 confirmed by FlyBase alignment of dbGSS accession AQ073846 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(3)3238
(Morris et al., )
P{EP}CRMPEP3238
(FlyBase, 2005, FlyBase, 1992-, )
P{EP}EP3238
(FlyBase, 1992-, Rawls, 2006)
P{EP}EP3238EP3238
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 12 )
Research paper
Morris et al., 2012, Genetics 191(4): 1227--1238
Divergent Functions Through Alternative Splicing: The Drosophila CRMP Gene in Pyrimidine Metabolism, Brain, and Behavior. [FBrf0219149]
Rawls, 2006, Genetics 172(3): 1665--1674
Analysis of pyrimidine catabolism in Drosophila melanogaster using epistatic interactions with mutations of pyrimidine biosynthesis and beta-alanine metabolism. [FBrf0192211]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Pena-Rangel et al., 2002, Genetics 160(3): 1035--1050
A misexpression study examining dorsal thorax formation in Drosophila melanogaster. [FBrf0147055]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material
Morris et al., , Genetics 191(4): 2--3
Supporting Information, File S1. [FBrf0219346]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]