Reference Report

Reference
Citation	Rawls, J.M. (2006). Analysis of pyrimidine catabolism in Drosophila melanogaster using epistatic interactions with mutations of pyrimidine biosynthesis and beta-alanine metabolism. Genetics 172(3): 1665--1674. (Export to RIS)
FlyBase ID	FBrf0192211
Publication Type	Research paper
PubMed ID	16361227
PubMed Abstract	The biochemical pathway for pyrimidine catabolism links the pathways for pyrimidine biosynthesis and salvage with beta-alanine metabolism, providing an array of epistatic interactions with which to analyze mutations of these pathways. Loss-of-function mutations have been identified and characterized for each of the enzymes for pyrimidine catabolism: dihydropyrimidine dehydrogenase (DPD), su(r) mutants; dihydropyrimidinase (DHP), CRMP mutants; beta-alanine synthase (betaAS), pyd3 mutants. For all three genes, mutants are viable and fertile and manifest no obvious phenotypes, aside from a variety of epistatic interactions. Mutations of all three genes disrupt suppression by the rudimentary gain-of-function mutation (r(Su(b))) of the dark cuticle phenotype of black mutants in which beta-alanine pools are diminished; these results confirm that pyrimidines are the major source of beta-alanine in cuticle pigmentation. The truncated wing phenotype of rudimentary mutants is suppressed completely by su(r) mutations and partially by CRMP mutations; however, no suppression is exhibited by pyd3 mutations. Similarly, su(r) mutants are hypersensitive to dietary 5-fluorouracil, CRMP mutants are less sensitive, and pyd3 mutants exhibit wild-type sensitivity. These results are discussed in the context of similar consequences of 5-fluoropyrimidine toxicity and pyrimidine catabolism mutations in humans.
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Secondary IDs	FBrf0190764
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (1)
	Df(3R)noi-B
Alleles (20)
	CRMP^+t10.8 CRMP^EP3238 CRMP^supA4 CRMP^supB3 CRMP^supI2 CRMP^supI3 pyd3^+t3.8 pyd3^La2 pyd3^Lb5 pyd3^Lb10 r^C r^Su(b).cSa Rheb^t10.8 su(r)¹ su(r)^+t7.3 su(r)^C4 su(r)^C5 su(r)^C11 su(r)^C43 w^+mC
Constructs (4)
	P{PYD1+} P{PYD2+} P{PYD3+} P{r^Su(b).cSa}
Genes (7)
	CG12118 CRMP pyd3 r Rheb su(r) w
Insertions (3)
	P{EP}CRMP^EP3238 P{EPgy2}CG12118^EY04394 P{GawB}NP2343
Natural transposons (1)
	P-element