A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}Rpd3EP3672 Insertion

General Information
Symbol Dmel\P{EP}Rpd3EP3672 Species D. melanogaster
Name FlyBase ID FBti0011756
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) Rpd3, Src64B Viability / fertility
Causes allele(s) Rpd3EP3672, Src64BEP3672 Stock availability none publicly available
LINE ID EP(3)3672
Genomic Location
Chromosomal location 3L ( 64B12 ) Sequence location 3L:4,625,577..4,625,577 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0132528 FBti0066126 FBti0041164 FBti0053606 FBti0115599 FBti0070899 FBti0058956 FBti0069273 FBti0055826 FBti0077005 FBti0102576 FBti0104520 FBti0125522_2 FBti0066609 FBti0125522_1 FBti0112196 FBti0029982 FBti0065041 FBti0057712 FBti0011756 FBti0072272 FBti0144005 FBti0052132 FBti0043638 FBti0067851 FBti0021601 FBti0068171 FBti0046331 FBti0125523_2 FBti0125523_1 FBti0005513_2 FBti0005513_1 FBti0100119 FBti0108259 FBti0044828 FBti0047348 FBti0105267 FBti0052692 FBti0068142
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:4,625,577..4,625,577 [+]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
64B12 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
64B17-64B17 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073107
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Rpd3
(Fernandez-Funez et al., 2000, Gene Disruption Project members, 2001-, Burgio et al., 2008)
Src64B
(BDGP Project Members, 2000-)
hide Alleles and Phenotypes
Causes alleles
Rpd3EP3672
(Fernandez-Funez et al., 2000, Gene Disruption Project members, 2001-, Burgio et al., 2008)
Src64BEP3672
(Gene Disruption Project members, 2001-)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Expression of Rpd3EP3672 under the control of Scer\GAL4GMR.PF has no affect on the eye.
(Mueller et al., 2005)
The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is not modified if the flies are also carrying Rpd3EP3672.
(Shulman and Feany, 2003)
Expression of Rpd3EP3672 under the control of Scer\GAL4GMR.PF does not result in a rough eye phenotype.
(Shulman and Feany, 2003)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(3)3672
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 3L:4606423-4606424 determined by FlyBase alignment of dbGSS accession AQ073107 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP3672
(Burgio et al., 2008)
P{EP}EP3672
(FlyBase, 1992-)
P{EP}Rpd3EP3672
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 12 )
Research paper
Burgio et al., 2008, PLoS Genet. 4(6): e1000089
Genetic identification of a network of factors that functionally interact with the nucleosome remodeling ATPase ISWI. [FBrf0211659]
Mueller et al., 2005, Genetics 171(3): 1137--1152
Genetic modifier screens on hairless gain-of function phenotypes reveal genes involved in cell differentiation, cell growth and apoptosis in Drosophila melanogaster. [FBrf0190751]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Shulman and Feany, 2003, Genetics 165(3): 1233--1242
Genetic modifiers of tauopathy in Drosophila. [FBrf0167629]
Fernandez-Funez et al., 2000, Nature 408(6808): 101--106
Identification of genes that modify ataxin-1-induced neurodegeneration. [FBrf0131287]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]