Recombinant construct P{cry-GAL4.E}

General Information
Symbol	P{cry-GAL4.E}	FlyBase ID	FBtp0013122
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	3 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Emery et al., 2000)
Uses	CV term Qualifiers & info Reference characterization cry (Emery et al., 2000)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
Allele	Scer\GAL4^cry.PE
Reference(s)	(Emery et al., 2000, Klarsfeld et al., 2004, Glossop et al., 2003, Allada et al., 2003, Stoleru et al., 2005, Picot et al., 2007, Grima et al., 2004, Stoleru et al., 2004, Zhao et al., 2003, Yoshii et al., 2008, Shafer et al., 2008, Johard et al., 2009, Picot et al., 2009, Helfrich-Forster et al., 2007, Daniels et al., 2008, Lear et al., 2005, Shafer et al., 2006, Hodge and Stanewsky, 2008, Lear et al., 2009, Yoshii et al., 2010, Nicolaï et al., 2010, Chen et al., 2011, Im and Taghert, 2010, Richier et al., 2008, Damulewicz and Pyza, 2011, Zheng et al., 2008, Grima et al., 2012, Kaneko et al., 2012)
Molecular data	Construct: The whole upstream region with the first intron and part of the second exon is fused in frame with the Scer\GAL4 coding region. (Emery et al., 2000)
Phenotypic class	circadian behavior defective, with W^Scer\UAS.cZa (Yoshii et al., 2010, Kaneko et al., 2012) circadian rhythm defective, with Clk^Scer\UAS.cZa (Zhao et al., 2003) circadian rhythm defective, with Shaw^{RA.Scer\UAS.T:Zzzz\FLAG} (Hodge and Stanewsky, 2008) locomotor rhythm defective (Shafer et al., 2008) locomotor rhythm defective, with Clk^Scer\UAS.cZa (Zhao et al., 2003) locomotor rhythm defective, with Cul-3^NIG.11861R (Grima et al., 2012) locomotor rhythm defective, with Scer\GAL80^Pdf.PS, W^Scer\UAS.cZa (Stoleru et al., 2004) locomotor rhythm defective, with Shaw^{RA.Scer\UAS.T:Zzzz\FLAG} (Hodge and Stanewsky, 2008) locomotor rhythm defective, with W^Scer\UAS.cZa (Stoleru et al., 2004, Kaneko et al., 2012) viable, with Clk^Scer\UAS.cZa (Zhao et al., 2003) viable, with Cul-3^NIG.11861R (Grima et al., 2012)
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage pacemaker neuron (Daniels et al., 2008) s-LNv neuron (Daniels et al., 2008) larval DN1 neuron (Daniels et al., 2008) adult stage DN1p neuron \| subset (Shafer et al., 2006) DN3 neuron \| subset (Shafer et al., 2006, Helfrich-Forster et al., 2007) DN1a neuron (Shafer et al., 2006) s-LNv neuron (Shafer et al., 2006, Yoshii et al., 2008, Damulewicz and Pyza, 2011) l-LNv neuron (Shafer et al., 2006, Yoshii et al., 2008, Damulewicz and Pyza, 2011) LNd neuron (Shafer et al., 2006, Yoshii et al., 2008, Damulewicz and Pyza, 2011) Pdf negative s-LNV neuron (Helfrich-Forster et al., 2007) LN Pdf neuron (Helfrich-Forster et al., 2007) DN1 neuron \| subset (Helfrich-Forster et al., 2007) DN3 neuron (Damulewicz and Pyza, 2011) DN1 neuron (Damulewicz and Pyza, 2011) medulla \| restricted (Damulewicz and Pyza, 2011) lamina \| restricted (Damulewicz and Pyza, 2011) lobula \| restricted (Damulewicz and Pyza, 2011)
Additional Information
Statement Reference Expression is within all LN neurons, a subset of the DN1 and DN3 neurons, and not at all in the DN2 neurons. Expression was not seen in the third instar larval Pdf negative s-LNv neuron. Fibres of the Pdf negative s-LNV neuron were observed to project towards the accessory medulla and arborize in its central area. (Helfrich-Forster et al., 2007) Scer\GAL4[cry.PE] is expressed in all of the LNv, LNd, DN3 and DN1 neurons in the protocerebrum. It also labels the medulla projections of LNv and DN3 and a lamina projection from the fifth s-LNv PDF-negative neuron. The staining intensity in the cell bodies in the accessory medulla was stronger in the l-LNv than in the s-LNv cells. There is also staining in restricted domains in the lobula which did not originated from the neurons that projected to the medulla. (Damulewicz and Pyza, 2011)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	cry13-GAL4 (Im and Taghert, 2010) cry13-Gal4 (Kaneko et al., 2012) Cry(39)-Gal4 (Shafer et al., 2008) cry-GAL4 (Grima et al., 2004, Emery et al., 2000, Nicolaï et al., 2010, Hodge and Stanewsky, 2008) cryGal4 (Glossop et al., 2003) cry-Gal4 (Chen et al., 2011, Picot et al., 2007, Daniels et al., 2008, Richier et al., 2008) cry-gal4 (Klarsfeld et al., 2004, Picot et al., 2009, Yoshii et al., 2008, Helfrich-Forster et al., 2007, Yoshii et al., 2010, Grima et al., 2012) Cry-Gal4 (Johard et al., 2009) cryGAL4-13 (Lear et al., 2009) cry-gal4(39) (Damulewicz and Pyza, 2011) cry-gal4¹³ (Shafer et al., 2006) crygal4³⁹ (Shafer et al., 2006) crypiGAL4 (Zhao et al., 2003, Allada et al., 2003, Zheng et al., 2008) crypi-GAL4-13 (Lear et al., 2005) P{cry-GAL4.E} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 30 )

Generate a list of	All references relating to this transgenic transposon ( 30 )

List References by type	Research paper ( 28 ) Personal communication to FlyBase ( 2 )
Recent research papers ( 4 )
	Grima et al., 2012, PLoS Biol. 10(8): e1001367 CULLIN-3 Controls TIMELESS Oscillations in the Drosophila Circadian Clock. [FBrf0219137] Kaneko et al., 2012, Curr. Biol. 22(19): 1851--1857 Circadian rhythm of temperature preference and its neural control in Drosophila. [FBrf0219631] Chen et al., 2011, Curr. Biol. 21(9): 719--729 QUASIMODO, a Novel GPI-Anchored Zona Pellucida Protein Involved in Light Input to the Drosophila Circadian Clock. [FBrf0213611] Damulewicz and Pyza, 2011, PLoS ONE 6(6): e21258 The Clock Input to the First Optic Neuropil of Drosophila melanogaster Expressing Neuronal Circadian Plasticity. [FBrf0214354] Show all research papers ...