Reference Report

Reference
Citation	Im, S.H., Taghert, P.H. (2010). PDF receptor expression reveals direct interactions between circadian oscillators in Drosophila. J. Comp. Neurol. 518(11): 1925--1945. (Export to RIS)
FlyBase ID	FBrf0210547
Publication Type	Research paper
PubMed ID	20394051
PubMed Abstract	Daily rhythms of behavior are controlled by a circuit of circadian pacemaking neurons. In Drosophila, 150 pacemakers participate in this network, and recent observations suggest that the network is divisible into M and E oscillators, which normally interact and synchronize. Sixteen oscillator neurons (the small and large lateral neurons [LNvs]) express a neuropeptide called pigment-dispersing factor (PDF) whose signaling is often equated with M oscillator output. Given the significance of PDF signaling to numerous aspects of behavioral and molecular rhythms, determining precisely where and how signaling via the PDF receptor (PDFR) occurs is now a central question in the field. Here we show that GAL4-mediated rescue of pdfr phenotypes using a UAS-PDFR transgene is insufficient to provide complete behavioral rescue. In contrast, we describe a approximately 70-kB PDF receptor (pdfr) transgene that does rescue the entire pdfr circadian behavioral phenotype. The transgene is widely but heterogeneously expressed among pacemakers, and also among a limited number of non-pacemakers. Our results support an important hypothesis: the small LNv cells directly target a subset of the other crucial pacemaker neurons cells. Furthermore, expression of the transgene confirms an autocrine feedback signaling by PDF back to PDF-expressing cells. Finally, the results present an unexpected PDF receptor site: the large LNv cells appear to target a population of non-neuronal cells that resides at the base of the eye.
DOI	10.1002/cne.22311
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Comp. Neurol.
Title	Journal of Comparative Neurology
Publication Year	1911-
ISBN/ISSN	0021-9967
Data from Reference
Alleles (18)
	Ecol\lacZ^Scer\UAS.cUa Pdf⁰¹ Pdfr³³⁶⁹ Pdfr⁵³⁰⁴ Pdfr^Scer\UAS.cMa Pdfr^T:Hsap\MYC Scer\GAL4^Aph-4-c232 Scer\GAL4^cry.PE Scer\GAL4^cry.PZ Scer\GAL4^dimm-929 Scer\GAL4^P2.4.Pdf Scer\GAL4^Pdfr.A Scer\GAL4^Pdfr.B Scer\GAL4^Pdfr.C Scer\GAL4^Pdfr.D Scer\GAL4^Pdfr.F Scer\GAL4^tim.PE Scer\GAL4^tim.Scer\UAS
Constructs (13)
	P{cry-GAL4.E} P{cry-GAL4.Z} P{GAL4-tim.E} P{Pdf-GAL4.P2.4} P{Pdfr-GAL4.A} P{Pdfr-GAL4.B} P{Pdfr-GAL4.C} P{Pdfr-GAL4.D} P{Pdfr-GAL4.F} P{Pdfr-myc} P{UAS-lacZ.U} P{UAS-pdfr.M} P{UAS-tim-GAL4}
Genes (6)
	Ecol\lacZ Pdf Pdfr per Scer\GAL4 T:Hsap\MYC
Insertions (4)
	P{CaryP}attP2 P{GawB}Aph-4^c232 P{GawB}dimm⁹²⁹ P{Pdfr-myc}attP2
Natural transposons (1)
	P-element
Polypeptides (1)
	Pdfr^T:Hsap\MYCPA
Transcripts (1)
	Scer\GAL4^Pdfr.BRA