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General Information
Symbol
Df(2R)enE
Species
D. melanogaster
Name
FlyBase ID
FBab0022271
Feature type
Also Known As
Df(2R)enE, enE, Df(enE), Df(2)enE, Df(2L)enE, Df(2R)en-E
Sequence coordinates
2R:11,496,174..11,496,174 (Df(2R)en[E]:bk1)
2R:11,537,511..11,537,511 (Df(2R)en[E]:bk2)
Member of large scale dataset(s)
Nature of Aberration
Gene Deletion and Duplication Data
Genes Deleted / Disrupted
Genes NOT Deleted / Disrupted
Complementation Data
 
Molecular Data
 
Genes Duplicated
Complementation Data
Completely duplicated
Partially duplicated
Molecular Data
Completely duplicated
Partially duplicated
Genes NOT Duplicated
Complementation Data
 
Molecular Data
 
Affected Genes Inferred by Location
Phenotypic Data
In combination with other aberrations
NOT in combination with other aberrations

The boundary between the maxillary and mandibular segments is unaffected in Df(2R)enE embryos.

Homozygous clones result in almost complete loss of the ocellar triangle.

Clones induced in the A compartment of the abdominal tergites appear completely normal, except that clones never make cuticle of the a6 type i.e. mutant cells in a6 territory make a5 cuticle. Clones induced during the larval period in the P compartment are always transformed into A cells, and form A-type cuticle. This cuticle tends to sort from the P compartment and form rounded excrescences on the surface of the integument or float as vesicles within the abdomen. When in the P compartment the clones make cuticle of the a5 type. Clones induced during the pupal period in the P compartment give rise to a5 cuticle in the anterior region of the P compartment or a1 cuticle when near the posterior limit. When P clones are transformed to A clones in the abdomen they make cuticle of the nextmost segment back. Clones induced in the pupa have divergent polarities. Anteriorly in the P compartment clones have normal polarity but in the posterior two-thirds of the P compartment the clones have reversed polarity (which shows a little non-autonomy). Cells in the A compartment which are simultaneously mutant for Df(2R)enE and smo3 behave like smo3 clones: they transform a6, a5 and a4 into a3, and a1 into a2. Cells in the anterior P compartment which are simultaneously mutant for Df(2R)enE and smo3 form a3 cuticle and bristles. Cells in the posterior P compartment which are simultaneously mutant for Df(2R)enE and smo3 form a2 cuticle and bristles. Double mutant clones of ptc16 and Df(2R)enE induced in the a2 region of the A compartment behave as ptc16 clones and make a1 cuticle. In the rest of the compartment clones make dusky a5 as opposed to clear a6-type cuticle. No clones make P-type cuticle. Single clones of ptc16 and Df(2R)enE in the A compartment can make both a1 and a5 cuticle, and reorganise polarity within and behind the clone. The results are slightly different for A6 than for more anterior segments: cuticle made by clones resembles the lightly pigmented cuticle found just posterior to a5 in segment A6. Pupal mutant clones of Df(2R)enE in the anterior part of P produce dusky a5 pigmentation and bristles, while further back the clones are lighter in colour, like a3. At the front of the P compartment polarity of clones is normal, while at the back it is reversed.

Clones mutant for ptc16 and Df(2R)enE induced in the posterior domain of the anterior compartment of the abdominal tergite produce a5 cuticle. Clones induced before 12 hours APF reveal that the mutant cells have different affinities than those around them, judging from the shape of, and cell density within, the clone. This different affinity is graded across the segment. In the posterior domain of the A compartment, the later the induction, the further anterior the clone, and the more anterior the clone, the more circular its shape and smaller its size. In the posterior domain of the A compartment twin clones are oriented with mutant posterior to wild type. In the anterior domain of the A compartment twin clones are oriented with mutant anterior to wild type. Some clones span both anterior and posterior domains of the A compartment. Clones mutant for ptc16 and Df(2R)enE induced in the posterior compartment of the abdominal tergite in the larval period or earlier rarely survive. Survivors are anteriorly located, produce a5 cuticle, and most fuse with the anterior compartment, a5 cuticle. Clones mutant for ptc16 and Df(2R)enE induced in the posterior compartment in the pupal period produce a5 cuticle and lie anterior to the twin clone. The clone and its twin can be separated by several cell diameters. The mutant clone is more circular and smaller that the twin. Clones mutant for ptc16 and Df(2R)enE induced in the posterior compartment in the pupal period after 12 hours APF are small and only partially transformed to A identity. Clones mutant for smo3 and Df(2R)enE induced in the posterior compartment of the abdominal tergite make a3 or a2 cuticle. The small number of clones produced after clone formation in the embryo tend to be elliptical with smooth boundaries. Clone induction in the larva produces mutant clones with less pigment and small bristles. These cells survive better than Df(2R)enE clones, especially in the posterior part of the P compartment. The boundaries of these clones are smooth. Clones mutant for smo3 and Df(2R)enE induced in the posterior compartment of the pleura form a3 or a2 cuticle embedded in the A compartments, having been ejected from the posterior compartment.

Homozygous embryos have duplicated RP2 neurons in several hemisegments.

Mutant clones cause posterior to anterior transformation in the adult female terminalia (ectopic gonopod thorn bristle) and in the adult male terminalia (ectopic lateral plate bristles).

Agam\enen-12A-5R rescue causes severe defects in the posterior compartments of the wings, both sexes are sterile. Agam\enen-10B-5 rescue causes slight defects in the posterior compartments of the wings, both sexes are fertile.

Homozygous embryos are smaller than wild type and display some alternating naked and denticulate cuticle. Df(2R)enE wgl-17 embryos are small, spherical, carry a lawn of unpolarised denticles (A cell type denticles), have no Keilin's organs (presumably due to lack of functional parasegment boundaries) and the thoracic and abdominal identities are differentiated. Scer\GAL4arm.PS mediated expression of wgUAS.cLa in wgl-17 Df(2R)enE embryos does not rescue segmentation, the embryos lengthen only a little and no Keilin's organs form. Instead of a lawn of denticles the ventral abdomen now makes naked cuticle. The T1 becomes largely covered by fine denticles normally found in the beard, the beard is not small and localised. Scer\GAL4arm.PS mediated expression of both wgUAS.cLa and enUAS.cGa in wgl-17 Df(2R)enE embryos causes a small beardless, near-spherical and unsegmented embryo with extruded organs (believed to be foregut and hindgut), i.e. less phenotypic rescue than for Scer\GAL4arm.PS mediated expression of wgUAS.cLa alone. Scer\GAL4arm.PS mediated expression of wgl-12.UAS in wgl-17 Df(2R)enE embryos at varying temperatures can be used to study the dose response to P{en1}wgen11 for A cells. At 17.5oC wgl-12.UAS produces the same phenotype as when wild type P{en1}wgen11 (wgUAS.cLa) is added. At 20oC there are only a few ventral denticles and some beard in T1. At 22oC there are two lateral stripes of ventral denticles and some beard in T1. At 23oC the abdomen is completely covered in denticles but there is no beard in T1. At 25oC the abdomen is completely covered in denticles, weak thoracic denticles are present, but there is no beard in T1. At 28.5oC embryos are indistinguishable from wgl-17 Df(2R)enE embryo. Scer\GAL4arm.PS mediated expression of both wgUAS.cLa and hhUAS.cIa in wgl-17 Df(2R)enE embryos causes naked cuticle to form in the place of the T1 beard. Scer\GAL4prd.RG1 mediated expression of wgUAS.cLa in wgl-17 Df(2R)enE embryos causes a lengthening of the whole embryo and instead of a lawn of denticles alternate stripes of naked and denticulate cuticle appear. Two types of denticle form in each band, small ones on the outside and larger ones inward. Individual denticles tend to point towards the nearest naked domain.

Mutant embryos show almost complete loss of naked cuticle. The mouthparts and posterior spiracles are poorly formed, and there is an early abnormal eversion of the mouthparts. The central and peripheral nervous systems are grossly disorganised.

Embryos fail to complete embryogenesis: defects are more extreme than for simple en mutant, en8. Clones in the wing are often associated with bulges in the disc epithelium. Clones transform posterior into anterior cells and duplicate both anterior and posterior structures.

Posterior wing compartments containing several Df(2R)enE clones appear to be abnormally large and neighboring wild type tissue has extra folds.

Clones in the posterior wing are abnormal, they differentiate ectopic dorsal vein tissue and sensilla. At the wing margin posterior clones cause cell autonomous transformation of posterior margin bristles into anterior bristles. Clones anterior to wing margin 3 are normal. Clones between veins 3 and 4 narrow the space between the veins and transform the margin bristles into innervated bristles. Posterior clones can induce overgrowth and ectopic venation in the surrounding wild type tissue. hhbar3 in combination with Df(2R)enE/en1 causes a phenotype reminiscent of a weak dpp mutation is produced, narrowing of the space between wing veins 3 and 4. Posterior wing clones affect adjacent cells of the anterior compartment and cause an overall reduction in wing size. Df(2R)enE ptc9 clones rescue the cell proliferation defect of Df(2R)enE clones, proliferation is induced both within the clones (posterior and anterior) and in surrounding wild type cells.

Stocks (1)
Notes on Origin
Discoverer

Ali and Kornberg.

 
Balancer / Genotype Variants of the Aberration
 
Separable Components
 
Other Comments
 

Results suggest en regulatory DNA can activate Agam\eninv-12A or Agam\enen-12A-5R expression by a transvection phenomenon leading to phenotypic rescue.

Deletes DNA from between 9kb upstream of and 50kb downstream of the en transcription unit, removing the entire en transcription unit and the C-terminal three exons of inv.

Synonyms and Secondary IDs (13)
References (64)