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FB2026_02
,
released June 18, 2026
[62D7-62D7];[62E5-62E5];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
62E1-62E2;62E5-62E6
62D7;62E5
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Df(3L)Spn-Δ3.1/Df(3L)BSC116 mutants die during pupal stages. Larval neuromuscular junctions in these mutants show an increase in active zone scaffold densities, and these are smaller; there is an increase in the frequency of spontaneous synaptic vesicle release in the presynaptic motoneuron, and a decrease in synaptic vesicle release evoked by single action potentials, as compared to controls; but average NMJ size is unchanged.
Df(3R)RhoGAP100Fex3.4/+ suppresses the neuromuscular junction phenotypes seen in Df(3L)Spn-Δ3.1/Df(3L)BSC116 mutants.
Lethal in combination with Df(3L)Aprt-32. Lethal in combination with Df(3L)R-G7. Inferred to overlap with: Df(3L)Aprt-32. Inferred to overlap with: Df(3L)R-G7.
Presence of P+PBac{XP5.RB3}BSC116 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(3L)BSC116 predicted from Release 3 genomic coordinates of PBac{RB}CG9004e00847 and P{XP}Spnd06681 are 62D7;62E6; however, polytene chromosome squashes showed the breakpoints 62E1-2;62E5-6. (The deletion forms a fusion band composed of part of the 62E1,2 doublet and all or part of 62E6.)